Expression And Significance Of BFGF And TGFβ₁ Protein In Different Periods Pathological Scar

Objective To study the expression of bFGF,TGFβ1 in different periods pathological scars and to investigate its role and its probable mechanism in the pathogenesis of scars.Methods Immunohistochemiscal techuique was performered to detect the expression and distribution of bFGF,TGFβ1 in pathological scars(43 cases) and normal skin(13 cases),statistics was used to analyze the data.Results The expressions of bFGF in proliferative phase scar were much stronger than that in maturation phase scar and the expressions of bFGF in pathological scar were much lower than that in maturation phase, there were significant difference between them (p<0.05); the expressions of TGFβ1 were no statistically different between proliferative phase scar and maturation phase scar (p>0.05), the expressions of TGFβ1 in pathological scar were much stronger than that in maturation phase (p<0.05).Conclusion The result indicated that the bFGF might play an important role in the pathogenesis of pathological scar; and TGFβ1 might be important endogenous growth factor which in sustain morphous and structure. Objective: To observe the effects of artesunate, hirudin, margarita liquid on fibroblast secretion , basic fibmblast growth factor(bFGF) , transforming growth factorβ1 (TGFβ1)derived from hyperolastic scar and keloid and to investigate the role of artesunate, hirudin, margarita liquid on the biological activity of fibroblasts derived from abnormal scars, as well as the mechanism by which medicamentum affects scars。Methods: 1,The dermal fibroblasts were cultured in vitro. This experiment were divided into two groups: human scar dermal fibroblasts group and human normal dermal fibroblasts group. Two groups were propagated in vitro model with artesunate, hirudin, margarita liquid for 24 hours. The human mRNA levels of bFGF , TGFβ1 in fibroblasts were determined by reverse transcription-fliorescence quantitative-polymerase chain reaction.2,The dermal fibroblasts were cultured in vitro. This experiment were divided into two groups: human scar dermal fibroblasts group and human normal dermal fibroblasts group. Two groups were propagated in vitro model with artesunate, hirudin, margarita liquid for 24 hours. The human proteic substance levels of bFGF , TGFβ1 in fibroblasts were determined by immunocytochemistryResults: 1,Real-time RT-PCR and Immunocytochemistry result showed that artesunate, hirudin, caused statistically increase in the human mRNA and the human proteic substance levels of bFGF for normal and hypertrophic scar fibroblast cell lines(P <0.05).It also caused statistically decrease in the human mRNA and the human proteic substance level of TGFβ1 for normal and hypertrophic scar fibroblast cell lines(P <0.05).2,margarita liquid not caused statistically increase in the human mRNA and the human proteic substance levels of bFGF for normal and hypertrophic scar fibroblast cell lines(P>0.05).It also not caused statistically decrease in the human mRNA and the human proteic substance level of TGFβ1 for normal and hypertrophic scar fibroblast cell lines(P>0.05).Conclusions: 1,artesunate, hirudin inhibits the secretion of TGFβ1 and facilitates the secretion of bFGF. Then it is used to regulate the growth ,proliferation,collagen secretion of fibroblasts derived from scars.2,margarita liquid can not inhibits the secretion of TGFβ1 and facilitates the secretion of bFGF. Then it is not used to regulate the growth ,proliferation,collagen secretion of fibroblasts derived from scars.