Construction Of Breast Cancer Sample Bank And Screening Of Serum Biomarkers For Breast Cancer

Breast cancer is one of the most common cancers found in women. In recent years, the occurrence rate grows rapidly in China, especially in developed regions. But there are only few effective biomarkers for early detection and prognosis prediction. Serum protomics provides a brand new and promising tool for the clinical biomarker searching. The serum proteomics aids the systematic study on the serum proteoms of heathy and sick conditions to find serum biomarkers for early detection and prognosis prediction.In this study, a well-parameter-controlled breast cancer sample bank was established. We used multiple methods to analyze mass spectrometry data of 21 serum samples from patients (7 of benign breast lump, 7 of lymph node negative breast cancer and 7 of lymph node positive breast cancer). Through analysis, we have selected A1BG and Mimecan as the candidates of biomarkers in serum, and they were further verified by ELISA.