Association Of Polymorphisms Of ANP And BNP Genes With Essential Hypertension

Objective To investigate the relationships between atrial natriuretic peptide (ANP) rs5065 and brain natriuretic peptide(BNP) rs198389 gene polymorphisms and human essential hypertension(EH).Methods A total of 976 individuals with EH and 9676 age- and sex- frequency matched normotensive(NT) control subjects were collected. The epidemiology data were gathered by mended Inter-heart questionnaire interview, and detecting blood glucose(FBG),total cholesterol(TC) and triglyceride(TG) by the method of biochemistry, then the ANP rs5065 and BNP rs198389 polymorphism was genotyped by real-time quantitative polymerase chain reaction (real-time Q-PCR) method. Then, the genetypes were analyzed by software of SDS 2.2.1. Hardy-Weinberg law was used to detect population representive and logistic regression analyses were used to investigate the relationships between the ANP rs5065 and BNP rs198389 gene polymorphism with essential hypertension.Results In all investigated population, frequencies of A and G Alleles of ANP rs5065 were 99.2% and 0.8%, respectively; frequencies of A and G Alleles of BNP rs198389 were 84.3% and 15.7%, The observed and expected genotypes were in agreement with the predicted Hardy-Weinberg equilibrium values (P=0.079;P=0.277). The frequency distribution of AA and AG+GG genotypes,A and G allele of BNP rs198389 was significantly different between the EH and NT group (P<0.05). The frequencies of AG+GG genotype and G allele were significantly higher in the EH group (30.5%和17.3%) than in the NT group (26.1%和14.1%); However, there is no difference of distribution of ANP rs5065 polymorphism between EH and NT group(P>0.05). By unconditional Logistic regression analysis of rs198389, AG+GG genotype presented a significantly higher risk of EH (OR=1.34, 95%CI=1.01-1.75, P=0.04) than AA genotype, this risk was still significant after adjusted for the EH risk factors such as age, sex, smoking, drinking, body mass index, triglycerides, and family history of EH(OR=1.63, 95%CI=0.89-3.00, P=0.12). G allele had more significantly increased risk of EH than A allele (OR=1.21, 95%CI=1.02-1.44, P=0.04). But there was no significant deference neither before nor after adjusted for the EH risk factors in unconditional Logistic regression analysis of rs198389.Conclusion The results suggest that the brain natriuretic peptide rs198389 polymorphism may be associated with EH, atrial natriuretic peptide rs5065 polymorphism may not be associated with EH.