Effects On Expression Of C-myc Gene Of HepG2 Cell And Inducement To Apoptosis Of HepG2 Cell By Inhibition Of ShRNA To SMYD3

AIM to employ the technology of shRNA to interfere expression of SMYD3 gene in HepG2 cell, to observe the expression of c-myc of HepG2 cell after SMYD3 gene decrease and the decrease effects to HepG2 cell apoptosis.METHODS to establish shRNA interfere plasmids for SMYD3 as 3 groups: Pgenesil- 1-s1 (with interfering effect), Pgenesil-1-s2 (with interfering effect) , negative contrast Pgenesil-1-hk (without interfering effect) and control group. The Lipofectmine2000 liposome (made at Invitrogen of USA) media-transfection is employed. RT-PCR was employed to detect the expression of SMYD3 gene and c-myc gene, before and after transfection. Flow cytometry (FCM) was employed to detect cell apoptosis of each group.RESULTS obvious expression of SMYD3 and c-myc gene in HepG2. Comparing with Pgenesil-1-hk group, the expression of SMYD3 gene mRNA are obviously inhibited in group Pgenesil-1-s1 and Pgenesil-1-s2,and a descend expression of c-myc gene mRNA are observed at same time in RT-PCR. The early apoptosis rate are significantly higher in group Pgenesil-1-s1 and Pgenesil-1-s2 than group Pgenesil-1-hk and control group.CONCLUSION SMYD3 and c-myc gene were detected obvious expression in HepG2 cell, the cells apoptosis is induced after the specifically dumbed expression of SMYD3 mRNA in HepG2 by interference of shRNA, and expression of that c-myc gene is inhibited.