The Relationship Between Human Osterosarcoma Cells And Voltage-gated Potassium Channel

Purpose: Growing evidences show that potassium channels play an important role in the development and growth of tumor. However, the participation of potassium channels in human osterosarcoma has not been well documented. In the present study, we explored the expression of potassium channels and the effects of potassium channel blockers on the proliferation and the cell cycle in human osterosarcoma cells.Method:The expressions of potassium channels, including kv1.1, kv1.3, kv1.5, kv2.1, kv3.3/3.4, kv4.1, kv5.1, kv9.3, herg, heag and kcnq1, were measured by RT-PCR in three human osteosarcoma cell lines of MG63,Saos-2 and SOSP-9607. Nonspecific kv channel blocker (4-AP and Cscl) and specific herg, heag and KCa channel blocker (E-4031, imipramine and TEA respectively ) were used to examine the functional role of potassium channels in the proliferation and cell cycle regulation by means of MTT and flow cytometry.Result:We detected the mRNA transcripts of kv1.3, kv1.5, kv2.1, kv3.3/3.4, kv4.1, kv9.3, herg and heag in all three osterosarcom cell lines, kv5.1 in MG63 and kcnq1 in SOSP-9607 and MG63 cells by RT-PCR. E-4031, imipramine and TEA had no significant effects on the proliferation and cell cycle. But 4-AP, at 3mM and 5mM, significantly suppressed the cell proliferation and arrested the cell cycle in G0/G1 phase. Cscl at 5mM also had a similar effects to 4-AP but minor.Conclusion:These results demonstrated that potassium channels are broadly expressed in the three human ostersarcoma cells MG63,Saos-2 and SOSP-9607 at transcript level. But only kv potassium channels involved in the proliferation and cell cycle regulation.