Establishment The New Homogeneous Methods For The Detection Of Serum Creatinine

Objective:To establish rapid, sensitive homogeneous assay of creatinine in serum.Methods:To establish biochemistry docimastic methodology and assessment technique by using of serum micromolecule substance acquisition technique,liquid enzymes stable technique,and appraise the different systems of homogeneous assay of creatinine in serum. Spectrophotometric homogeneous kinetic method and spectrophotometric homogeneous endpoint method had been established, which could be used in biochemistry autoanalyzer. Flourescence homogeneous method and chemoluminescence homogeneous method had also been established, which were applied to manual technique.Results:The liner range of the spectrophotometric homogeneous kinetic method for the detection of creatinine in serum is up to 3360μmol/L, the average rate of recovery is 100.8%,the within run and between run CV is 0.0071～0.0204 and 0.0086～0.0316, Comparing this method with the enzymatic colorimetry commended by the Clinical Laboratory Procedures, we obtained the regression equations: Y =1.01X-0.12,r=0.9945,The healthy fellow serum creatinine is 57～129μmol/L(male),41～105μmmol/ L(female).The liner range of the spectrophotometric homogeneous endpoint method for the detection of creatinine in serum is up to 8840μmol/L, the average rate of recovery is 100.3%,the within run and between run CV is 0.0065～0.0215 and 0.0079～0.0256, Comparing this method with the enzymatic colorimetry commended by the Clinical Laboratory Procedures, we obtained the regression equations: Y =0.970X+2.77, r=0.9954,The healthy fellow serum creatinine is 56～132μmol/L(male),41～109μmmol/ L(female).The liner range of the flourescence homogeneous assay method for the detection of creatinine in serum is liner in the range of 1280μmol/L, the average rate of recovery is 101.2%,the within run and between run CV is 0.0163～0.0341and 0.0236～0.0427, Comparing this method with the enzymatic colorimetry commended by the Clinical Laboratory Procedures, we obtained the regression equations: Y =1.020X-1.79,r=0.9907,The healthy fellow serum creatinine is 52～132μmol/L(male),37～109μmmol/ L(female).The liner range of the chemoluminescence homogeneous assay method for the detection of creatinine in serum is up to 1225μmol/L, the average rate of recovery is 100.2%,the within run and between run CV is 0.0095～0.0206and 0.0163～0.0329, Comparing this method with the enzymatic colorimetry commended by the Clinical Laboratory Procedures, we obtained the regression equations: Y =1.010X-1.36,r=0.9913,The healthy fellow serum creatinine is 53～133μmol/L(male),39～111μmmol/ L(female).Conclusion:Our study established four methods for the detection of creatinine in serum, including the spectrophotometric homogeneous kinetic method, the spectrophotometric homogeneous endpoint method, the flourescence homogeneous assay method and the chemoluminescence homogeneous assay method. Each method meet clinical practical requirements. These methods were selective, specific, sensitive and easily measured and so on . It has been proved that these new methods can meet the different levels of clinical laboratory requirements and suitable for clinical generalization and application.