The Association Between APM1 Gene Single Nucleotide Polymorphisms And Type 2 Diabetes In Chinese Han Population

Objective:It was reported that adiponectin could regulate glucose levels, lipid metabolism. And some single nucleotide polymorphisms (SNPs)in the adiponectin gene(APM1)were in association with type 2 diabetes because they might influence the gene expression or change the protein function. So the aim in our study is to investigate these associations between adiponectin gene SNPs and type 2 diabetes in Sichuan Chinese Han Population.Methods: In this study, a total of 798 subjects were recruited, consisting of 338 type 2 diabetic subjects and 460 non-diabetic subjects. There was no statistical significance in age and sex ratio between these two groups. All subjects were unrelated Chinese Han population in Sichuan province and selected from the West China Hospital, Sichuan University during the years of 2002-2006.we genotyped 6 known SNPs of APM1 gene(-11391G/A, -11377C/G, -10068G/A, G54V, Y111H and 4545G/C)by means of polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). The allelic distribution of each SNP was tested for the Hardy-Weinberg equilibrium using Hardy-Weinberg equilibrium caculater. Most calculations and statistical analyses were performed using the SPSS for Windows software (version 13.0; Chicago IL). The software SHEsis which is available online was applied to estimate haplotype frequencies and to compare their differences (http://analysis.bio-x.cn/myAnalysis.php).Results:(1),The results showed that there were no polymorphisms at the sites of -11391G/A, G54V and Y111H in Sichuan Chinese Han Population.(2),The frequency of -11377 GG genotype in diabetic patients was significantly higher than that in controls(P=0.03), and subjects with -11377 GG genotype had an increased risk of T2D(OR=2.13). After the T2D patients were subdivided into two groups (obese and non-obese group) on the basis of the Asia Pacific obesity criteria (1999). We found that -11377C/G variant was only associated with diabesity (odds ratio 2.45, P=0.02) in Logistic regression models, and 59% risk of diabesity could be attributed to -11377 GG genotype.(3),The frequency of 4545 CC genotype in non-obese diabetic patients was significantly higher than that in controls(P=0.004), and 4545 CC genotype individuals was at an higher risk of non-obese diabetes (OR=2.34). 57% of the risk is related to this polymorphism(4),For -10068G/A variant, there were no differences in allele and genotype frequencie(sP > 0.05)between type 2 diabetes patients and control subjects.(5),During these four haplotypes including polymorphisms -11377C/G and 4545G/C, the -11377G/4545C haplotype was related to diabesity (P=0.03, OR=2.12, 95%CI: 1.08 - 4.14).Conclusion:The present study suggests that -11377GG and 4545CC genotype may be susceptible to diabesity, non-diabesity respectively. However, there was no association between -10068G/A variant and type 2 diabetes. In addition, there were no polymorphisms in -11391G/A, G54V and Y111H in Sichuan Chinese Han People.