| Salvia przewalskii Maxim. is a plant of Subgenus Salvia of Salvia of Labiatae. As an herb distributed in the western areas of China, S. przewalskii has been utilized for a long historical time. The content of diterpene quinines of S. przewalskii is high, so its quality is good. Because of the plentiful resources, the prospect of research, development and utilization of S. przewalskii is very well. The aim of this topic is to study on the chemical constituents of S. przewalskii which have the enormous potential to treat glomerulonephritis. The isolation and purification of the chemical constituents from 50% ethanol extracts of S. przewalskii were carried out repeatedly by silica gel, Sephadex gel, ODS C18 and MCI gel column chromatography. And the structures of the compounds were identified by physical, chemical methods and spectral data of UV, IR, MS, 1H-NMR, 13C-NMR and 2D-NMR. Thirteen compounds were obtained and identified as tanshintriol A (SP-01), tanshintriol B (SP-02), isoganxinonic acid A (SP-03), ganxinoside A (SP-04), cryptotanshinone (SP-05), tanshinol B (SP-06), paeoniflorin (SP-07),γ-linolenic acid (SP-08), protocatechualdehyde (SP-09), protocatechuic acid (SP-10), caffeic acid (SP-11), rosmarinic acid (SP-12) and salvianolic acid B (SP-13), respectively. Compounds SP-01, SP-02, SP-03 and SP-04 are new compounds. Compounds SP-06 and SP-08 are obtained from S. przewalskii for the first time. Compound SP-07 is obtained from Labiatae for the first time. It is a significant contribution to study on the chemical constituents of S. przewalskii in order to find new compounds, to confirm the active constituents for treating glomerulonephritis, to interpret the material basis on pharmacological activity, to accumulate research data of the chemistry and pharmacology, to set up the standard of quality control and to research and develop the new drug.Polygonum capitatum Buch.-Ham. ex D. Don is a plant of Cephalophilon of Polygonum of Polygonaceae. As a'Miao'folk herb in Guizhou province of China, P. capitatum has diuresis, antiinflammatory and analgesis activity.'Relinqing capsule'and'Relinqing granule'made up from the raw material of P. capitatum have a significant effect in clinical application. The aim of this topic is to study on the chemical constituents of P. capitatum systemically. The isolation and purification of the chemical constituents from 80% ethanol extracts of P. capitatum were carried out repeatedly by silica gel, Sephadex gel, ODS C18 and MCI gel column chromatography. And the structures of the compounds were identified by physical, chemical methods and spectral data of UV, IR, MS, 1H-NMR, 13C-NMR and 2D-NMR. Fourteen compounds were obtained from the petroleum ether fraction. Seventeen compounds were obtained from the ethyl acetate fraction. Nine compounds were obtained from the n-butanol fraction. And these forty compounds were identified asβ-sitosterol (PC-01), tricosane (PC-02), linoleic acid (PC-03), hexadecanoic acid (PC-04), docosanoic acid (PC-05), lignoceric acid (PC-06), hexadecanoic-2,3- dihydroxypropyleste (PC-07), docosanoic-2,3-dihydroxypropyleste (PC-08), tricosanol (PC-09), pentacosanol (PC-10), oleanolic acid (PC-11), ursolic acid (PC-12), octacosa-1,27-diene (PC-13), docosyl ferulate (PC-14), emodin (PC-15), protocatechuic acid (PC-16), gallic acid (PC-17), ethyl gallate (PC-18), (+)-catechin (PC-19), (-)-epicatechin-3-O-gallate (PC-20), kaempferol (PC-21), quercetin (PC-22), kaempferol-3-O-α-L-rhamnopyranoside (PC-23), quercitrin (PC-24), kaempferol- 3-O-β-D-glucopyranoside (PC-25), hirsutrin (PC-26), 2″-O-galloyl quercitrin (PC-27), 2″-O-galloyl hirsutrin (PC-28), silybin (PC-29), 2,3-dehydrosilybin (PC-30), 2,3-dehydrosilychristin (PC-31), 3-O-galloyl-D-glucopyranoside (PC-32), 2-O-butyl-α-D-fructofuranoside (PC-33), 2-O-butyl-β-D-fructofuranoside (PC-34), 2-methoxyl- 1,4-benzenediol-4-O-β-D-glucopyranoside (PC-35), 2-methoxyl-1,4-benzenediol- 1-O-β-D-glucopyranoside (PC-36), 5-methoxyl-1,3-benzenediol-1-O-β-D-glucopyranoside (PC-37), 1,3-dimethoxyl-2,5-benzenediol-5-O-β-D-glucopyranoside (PC-38), salidroside (PC-39) and tryptophan (PC-40), respectively. Compounds PC-05, PC-06, PC-07, PC-08, PC-09, PC-10, PC-11, PC-12, PC-15, PC-19, PC-20, PC-23, PC-25, PC-28, PC-34, PC-35, PC-36 and PC-40 are obtained from P. capitatum for the first time. Compounds PC-13, PC-14 and PC-32 are obtained from Polygonum for the first time. PC-29, PC-30, PC-31, PC-33, PC-37, PC-38 and PC-39 are obtained from Polygonaceae for the first time. It is a significant contribution to study on the chemical constituents of P. capitatum in order to set up the quality standard of the drug crude materials and preparation, to screen active constitutents, to confirm pharmacological active constituents and to develop and utilize the resource of P. capitatum. It is very important that three flavonolignans are obtained from Polygonaceae for the first time in order to find the new resource of flavonolignan from plants, to interpret the biosynthetic path of flavonolignan and to investigate the genetic relationship between Polygonaceae and the other plant families. |
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