The Relationship Of Vascellum Cellular Adhesion Molecule-1 In Aterial Endothelialitric And Atumor Necrosis Factor Alpha,Interlenkin-1β In Myocardial Cell Of The Insulin Resistance Rat

ObjectiveThe model of insulin resistance was made by high-fat diet.The level of insulin was detected and the expression of VCAM-1 in Aterial Endothelialitric and Atumor Necrosis Factor Alpha,Interlenkin-1βwere observed in myocardial cell.To observe the change of VCAM--1,Atumor Necrosis Factor Alpha,Interlenkin-1βwhich caused the changing in high-fat diet rats,and to evaluate its action in diabetic cardiomyopathy accelerated by insulin resistance.methods20 male Wistar rats were randomly assigned into 2 groups:normal diet group and high-fat and high-suger diet group after 1-week-accommodation raising.Every rat was weight once a week,Each froup was fed for 8 weeks.IR was verified by euglycemic-hyperinsulinemia clamp technique,Myocardial tissue were taken for immunohistovhemistry detecion,Blood from left ventricle was taken for the detection blood serum's factors.Level of insulin was detemined and the expression of VCAM-1 in Aterial Endothelialitric and TNF-α,interlenkin-1βin myocardial cell was examined by immunhistochemistry.The differences of these factors between the two groups and the coorelation between the factors linked to IR and atherosclerosis were respectively analyzed by statistics softwate SPSS 13.0Result1.In high-fat and suger diet group,the VCAM-1 in Aterial Endothelialitric and Atumor Necrosis Factor Alpha,interlenkin-1βin myocardial cell were significantly expressed(P＜0.05).But in normal diet group,there is little expression. 2.Immunohistochemistry index:the expression of VCAM-1 was positive correlated with expression of TNF-α,interlenkin-1βin myocardial cells(p＜0.05)).Conclusions1.in insulin resitance,In high-fat and suger diet group,the VCAM-1 were significantly expressed in aterial endothelialitric,But in normal diet group,there is little expression.2.in insulin resitance,In high-fat and suger diet group,the Atumor Necrosis Factor Alpha were significantly expressed in myocardial cells,But in normal diet group,there is little expression.3.in insulin resitance,In high-fat and suger diet group,interlenkin-1βwere significantly expressed in myocardial cells,But in normal diet group,there is little expression.4.Immunohistochemistry index:the expressionof VCAM-1 in aterial endothelialitric was positive correlated with expression of Atumor Necrosis Factor Alpha in myocardial cells(p＜0.05).and the expression of VCAM-1 in aterial endothelialitric wad positive correlated with expression of interlenkin-1βin myocardial cells,too.(p＜0.05)...