| PrefaceThe incidence of brain injury and accompanied mutilation and mortality rates have increased year by year with the development of the economy and transportation.The abnormal expression of cytokines in traumatic brain injury after drug treatment was investigated to illuminate the mechanism of cerebral injury,and thus to direct the clinical treatment,which produces the elevated recovery rate and decreased mortality and mutilation rates.The IL-6 belongs to pro-inflammatory cytokine,which has pleiotropia biological activityafter brain injury and plays an important role in the secondary brain injury and in the protection of brain,the magnesia mixture currently has certain protection for traumatic brain injury demonstrated in many researches,but the mechanism has not been illuminated completely.In this study,the animal model for brain injury by the Feeney method of free falling object was established,and given magnesium sulfate.The changing in the concentration of IL-6 and brain edema in rats' brain tissue was observed to investigate it's double mechanism and the effect of the medication.Material and MethodsTotal 73 SD masculinity rats were included in this study,whose body mass were from 270g to 300g,and randomly divided into three groups:vulnerate control group(n=38),magnesium sulfate group(n=30) and normal control group(n=5).Each group was subdivided into six time points:1h,4h,8h,12h,24h and 4d after injury, there were 5 rats in every time point in vulnerate control group and magnesium sulfate group,and there were 5 rats in normal control group.Experimental rats were anaesthetized through injecting 10%chloral hydrate(3mg/kg) into abdominal cavities, and a diameter of about 5mm bony window was drilled in every rats in about 2mm to the right of the median line and about 2mm to the post of sutura coronalis.The device of Feeney free falling body was used to injury the rats.After injury,the vulnerate control group was immediately sutted and was not given drug,the magnesium sulfate group was given peritoneal injection 12.5%magnesium sulfate(300mg/kg),the normal group was given no treatment.The rats were put into death at the time of 1h,4h,8h,12h,24h and 4d respectively after injury,and to get brain tissues.Partial brain tissues were immediately put into 10%formaldehyde and made into wax sample after fixing; and partial brain tissues were reserved in refrigerator with temperature70℃below zero. The moisture in the pallium at the edge of contusion foci of rats was calculated according to Elliotl formula in therapeutic group and the control group,the contents of IL-6 were determined through ELISA method,and the sample was obtained for pathologic examination.Statistical analysis:all measurement data were expressed as (?)±s,and statistical significance was evaluated by multiple comparison of test and ANOVA.A P value of 0.05 or less was considered to indicate statistical significance. All statistical processes were completed via SPSS(V13.0).ResultsUnder the macroscope,obvious inflammatory reaction,cellular edema and inflammatory cell infiltrating were found after cerebral injury.The moisture of brain tissues at 1h,4h,8h,12h,24h and 4d after brain injury in rats was compared,and the increasing trend was obvious,after the increasing trend there was another decreasing trend(every two time points was compared in the six points,P＜0.05),and the moisture of brain tissue at 24h after injury was highest.After 4 days the moisture of brain tissue decreased obviously,Comparing the two interfering groups with normal control group, the moisture of brain tissues were still increased.Comparing the magnesium sulfate group with the vulnerate control group,the moisture of brain tissuedecreased obviously (P＜0.05),these results indicated that during 24h after brain injury of rats,brain edema was increased trend,and the result of the magnesium sulfate group was obvious in relieving brain edema.IL-6 in the vulnerate control group was in a double hump trend at the time of 1h,4h,8h,12h,24 hand 4d after injury(P＜0.05).The magnesium sulfate group could restrain the upgrading of IL-6 in the earlier period,the group could promote the expression of IL-6 in the advanced stage.Comparing the magnesium sulfate group with the vulnerate control group,the expression of IL-6 of the two groups diffed obviously(P＜0.05),The expression of IL-6 of the normal control group was normal,comparing this group with other groups,the result was obvious(P＜0.05),the result of this experiment indicated that the magnesium sulfate could effect the expression of IL-6,and played an important role in protecting the brain.ConclusionIn the acute phase of brain injury of rats in 24h,the concentration of IL-6 was in an increasing trend.As IL-6 increased,the brain edema was also aggravated obviously.Magnesium sulfate were used early to decrease the concentration of IL-6 and brain edema,The concentration of IL-6 decreased in the intermediate stage obviously(24h-4d),the concentration of IL-6 increased again in the advanced stage. Magnesium sulfate could increase the concentration of IL-6,which could promote the regeneration and reparation of the brain tissues. |
PDF Full Text Request