| Enteromorpha Prolifera is a kind of wild green algae in China, with its rich resource and nutrient, food and medicinal value. However, in recent years, the domestic study on Enteromorpha Prolifera especially on its polysaccharide has been few reported. The thesis focused on the extraction and purification technology of polysaccharide from it, as well as the analysis of its basic physical and chemical properties. The in vitro antioxidant capacity of polysaccharide was tested by using the scavenging capacity of ROS as indicator. Furthermore, the biological functions of polysaccharide including the antioxidant and hypolipidemic activity were investigated through animal trials.(1) Extracted polysaccharide from Enteromorpha Prolifera in hot water. The optima parameters for the extraction were determined through single factor experiment and orthogonal test as follows: extraction in hot water, temperature 90℃for 3h at the solid to liquid ratio1:40. The effects of extraction temperature and the time on the extraction content were significantly while the ratio of solid to liquid was not obvious.(2) The main composition of crude polysaccharide from Enteromorpha Prolifera (EP1) was tested as follows: saccharide 33.06%, protein 3.23%, and sulfate 15.85%. After the deproteinization with trichloroacetic acid, it had protein1.56% and saccharide 44.21%. The deproteinization rate reached 51.69%, and the content of saccharide increased by 33.72%.(3) The polysaccharide extracted from Enteromorpha Prolifera was deproteinized with trichloroacetic acid, and then was separated by Sephadex G-100. The component with homogeneous molecular weight of some protein was preliminarily deduced to be a complex of polysaccharide and protein which needs to be further confirmed.(4) The in vitro antioxidant properties of the polysaccharide were tested by using the capacity to scavenge ROS as indicators. The results showed that EP1 could scavenge O2 ? effectively. The maximal inhibition rate of 55.11% was given at the concentration of EP1 25 mg?mL-1, whereas the antioxdation activity of EP1 was lower than that of VC. EP1 had powerful capability to scavenge OH?,and the scavenging rate reached to 75.52% at the concentration of EP1 0.30 mg?mL-1. The capability to scavenge OH? of EP1 was similar to that of VC. And the more concentration of EP1 came out the more capability to scavenge OH?. The results showed that EP1 had powerful activities to scavenge ROS.(5) In a control study, the hyperlipidemia model by high fat diet was employed to test the inhibition activities of EP1 on hyperlipidemia of mice, by different EP1 ig treatments for 4 weeks. The results showed that: EP1 could remarkably reduce the content of TC, TG, increase the HDL-C in the serum of hyperlipemia mice, but had no significant effect on the change of LDL-C. The results revealed that EP1 had a certain efficacy for hyperlipidemia. The liver index of the treated mice was much higher than control, which indicated that EP1 can not inhibit the hepatomegaly of mice effectively.(6) By comparison the antioxidion indicators (MDA, T-SOD, T-AOC, T-AOC/MDA in serum and liver) of NG, HL and MEP through the hyperlipidemia experiment. The following are the results: EP1 could significantly inhibit the generation of MDA in serum and improve T-AOC,T-SOD in liver and T-AOC/MDA in serum, while had no significant effects on other indicators. EP1 could obviously reduce the degree of lipid peroxidation, had certain but not comprehensive effects to improve the total antioxidant capacity and optimize the antioxidant/oxidative balance in vivo.Extraction and purification technology, basic physical and chemical properties of polysaccharide in Enteromorpha Prolifera were studied in this thesis, and its anti-oxidation in vitro and in vivo, hypolipidemic activity were tested. The results provide the basic data and reference for further research on polysaccharide in Enteromorpha Prolifera and its application to food, health food or pharmaceutical industry. |
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