Effect Of Polysaccharid Extracted From Scutellaria Barbata D.Don Associate With CTX On Correlative Cytokines In Blood Serum In H22-Loading Mice

Objective:The thesis is to study effect of polysaccharid extracted from Scutellaria barbata D.Don associate with CTX on cytokines in blood serum in H₂₂-loading miceMethods:Mice bearing in vivo transplanted tumor—H₂₂ hepatic carcinoma were used.the experimental group is divide like this:control,the group which was transplanted tumor—H₂₂ but never was given drugs(negative control),groups which were given SBP,groups which was given CTX,groups which were given SBP associate with CTX.After lOdays,the mice were put to death to get serum to apply ELISA to detect the content of IL-2,IL-12,TNF-α,VEGF.Use SPSS15.0 to analyse the data.Results:â‘ Draw comparisons with negative control,the compounds of SBP can up-express IL-2,IL-12,TNF-αand down-express VEGFâ‘¡The compounds of SBP and CTX can up-express IL-2,IL-12,TNF-αand down-express VEGF.â‘¢Draw comparisons with negative control,the group which were given CTX(30mg/kg,20 mg/kg )showed that CTX can up-express IL-2.â‘£The group which were given CTX(30mg/kg,20 mg/kg,10 mg/kg)showed that CTX gave no effect on the content of IL-12,TNF-α,VEGF.⑤SBP associate with CTX can up-express IL-2,IL-12,TNF-αand down-express VEGF.Conclusion:â‘ The compounds of SBP can upexpress IL-2,IL-12,TNF-α.â‘¡CTX can upexpress IL-2,but had no effect on the content of IL-12,TNF-α,VEGF.â‘¢SBP associate with CTX can upexpress IL-2,IL-12,TNF-αand downexpress VEGF in H₂₂-loading mice.