The Research Of MicroRNA-20a Expression In Colorectal Carcinoma And Its Pathoclinical Relevance

Objective To detect the miR-20a expression in the colorectal carcinoma and normal colorectal tissue,and analyze the differences of miR-20a expression between the colorectal carcinoma and normal colorectal tissue,the clinical stages and the degree of differentiation.To investigate the mechanism of miR-20a in the process of the tissue malignant transformation.Method Collect 46 specimens of the colorectal carcinoma after operation,and detect the expression of miR-20a in the carcinoma tissue(T) and the normal colorectal tissue(N) by fluorescence in situ hybridization.Statistically analyze the relevance between the miR-20a expression and the pathoclinical characteristics.Results 1.The positive rate of miR-20a expression in colorectal carcinoma is 63.04%,and that of colorectal normal tissue is 23.91%.There is significant difference between them by the statistical analysis(P＜0.01).2.The positive rate of miR-20a expression in colorectal carcinoma of Dukes stage A+B,C and D period are 53.85%,78.57%and 66.67%,respectively.There is no statistical difference among the Dukes' staging groups of colorectal carcinoma(P＞0.05).3.The positive rate of miR-20a expression in the well differentiated, moderately differentiated and poorly differentiated colorectal carcinoma tissues are 66.67%and 77.27%and 44.44%,respectively.There is no statistical difference among the each differentiation group of colorectal carcinoma(P＞0.05).Conclusions 1.The miR-20a expression in the colorectal carcinoma is higher than that of the colorectal normal tissue.It points to that in the colorectal carcinoma, the miR-20a with high expression negatively regulate E2F-1 expression,and it leads to the loss of E2F-1-induced apoptosis and then the carcinoma continues to progress.2.There is no statistical difference among the miR-20a expression of Dukes staging groups and each differentiation group of colorectal carcinoma.It prompts that miR-20a may be an important regulatory factor in the process of tumorigenesis,while it is nothing to do with the progress and tumor differentiation.