| Osteoporosis(OP) is a disease which charactorised in bone density reduction, bone micro-structural damage with increased bone brittleness therefore more prone to bone fracture.However,the academic community has valued intervention of bone movement as a positive means of development.This experiment was aimed at understanding the different forms of movement of simulated weightlessness on the tail-suspended rats and its bone mechanism.Forty 6-month-old SD rats were divided into 5 groups:a controlled group to simulate the loss of the reorganization of the restoration of simulated weightlessness + free group,simulated weightlessness + swimming group,simulated weightlessness group + running group.The tibia cancellous bone histomorphometry indicators,femur and lumbar spine bone mineral density and bone metabolism in the blood concentration of related indicators of the rats in each group were determined after slaying the rats.Experimental period was 4 weeks;suspension time for 4 weeks;movement form of swimming and treadmill training 6 days/week.Purpose:1.The establishment of an ideal simulated weightlessness in rat tail-suspension model, the model of simulated weightlessness on bone mineral density and bone metabolism in the impact of the internal control mechanism.The rear suspension is osteoporosis in the present study a better model.Therefore,to establish and select the appropriate animal model to study the pathogenesis of osteoporosis,at the same time to find effective methods of prevention and treatment.2.To study the different forms of exercise movement in rats after simulated weightlessness on bone mass,bone metabolism and bone metrology organizations such as the role of the effect of the differences between the means of intervention at the same time the choice of sports and exercise prescription to provide the theoretical basis for the formulation.3.Explore the treadmill movement patterns and swimming patterns on bone mineral density in rats.4.Explore the treadmill movement patterns and swimming patterns of biochemical markers of bone metabolism affected. 5.To explore the treadmill movement patterns and swimming patterns of the bone tissue in rats the impact of metrology.Methods:1.The establishment of animal model of tail suspension device.Rat tail will be using medical adhesive tape wrapped around the roots scattered around other parts of the bear,in the light of Chen Jie,the model of the rat tail-suspension and improved methods,a reasonable suspension;With continuous growth in rats,it is necessary to continue to mediate a high degree of tail suspension.2.Tail-suspended rat model of response to the observation,observation of sporting activities during the rat,diet,weight change,skin color of the tail.3.Biochemical markers of bone metabolism measurement.Microplate reader and Nanjing,the company completed biological reagents measured serum calcium,phosphorus ions,alkaline phosphatase,tartrate-resistant acid phosphatase4.Rat femur bone mineral density measurementThe use of dual-energy-ray scanners detect rat femur bone mass,bone mineral density5.Tibial bone tissue measurement metrology.Removed after tibial fixation with 10%formaldehyde in 2 days,2 hours and water washing,dehydration alcohol gradient(75%,80%,90%,100%all day),methyl methacrylate-embedded,50μm slices(not part of grinding,direct observation of fluorescence),glue stick and remove chip code compression for 24 hours,fine sandpaper grinding,polishing alumina(5μm),HE staining,observed under the microscope and film.Results1.indicators of bone mineral density in the femur,swimming and treadmill rats compared with the loss of significant reorganization of the latter is greater than(P＜0.05);Sports and Freedom group have increased compared to the restoration group,and the treadmill group increased more significantly(P＜0.01).Indicators of bone mineral density in the spine,swimming group,treadmill group compared with the loss of reorganization than the latter(P＜0.05);the restoration of the exercise group and the Liberal group,significantly higher than the latter(P＜0.05). 2.calcium concentrations,simulating loss of the reorganization of higher than control group(P＜0.05);exercise group lost reorganization and simulation group has decreased significantly(P＜0.05);exercise group compared with the freedom of the restoration of a significant decrease(P＜0.05);Phosphorus concentration,the restoration of movement and freedom of group compared with the loss of the reorganization,there are significant differences(P＜0.05).ALP,lost control group compared to restructuring and a significant reduction (P＜0.05);exercise group compared with the simulated loss of reorgan-ization significantly increased(P＜0.05);treadmill group compared with the freedom to resume significant increase(P＜0.05)TRACP,missing reorganization compared with the control group increased significantly(P＜0.01);loss of reorganization with the treadmill group,free to resume a significant reduction in group(P＜0.01);swimming group and the freedom to restore group significantly increased(P＜0.01),the Running group and the freedom to run the restoration group decreased significantly(P＜0.05).3.The static parametersTrabecular bone volume(TBV(%)),significant loss of restructuring than quiet group(P＜0.01);exercise group lost significantly higher than that of recombinant (P＜0.05).Trabecular thickness(Tb.Th)lostless than the reorganization of a quiet group,but no statistical significance;treadmill group restructuring and losses,to restore freedom group significantly increased(P＜0.05).Trabecular number(Tb.N),lost less than the quiet group of recombinant(P＜0.05); swimming group and the Liberal loss of the restoration group was significantly higher than recombinant(P＜0.05);treadmill group was significantly higher than the loss of recombinant(P＜0.01);treadmill group compared with the freedom to resume significant increase(P＜0.01).Mean trabecular spacing(Tb.Sp),lost more than a quiet restructuring group(P＜0.05); the freedom to restore group,swimming group compared with the loss of the reorganization,are lower than the loss of recombinant(P＜0.05);treadmill group was significantly lower than the loss of recombinant(P＜0.01). Cortical thickness(MCT),lost significantly less than the reorganization of a quiet group(P＜0.05);running higher than the loss of the reorganization of the Running group,(P＜0.05);swimming group and the relative loss of freedom of the restoration group has increased the trend of restructuring.4.The dynamic ParametersPercentage of fluorescent-labeled perimeter(%L.Pm),higher than the control group lost recombinant(P＞0.05);relative simulated reorganization,significantly reduce the freedom of the restoration group(P＜0.05),the exercise group were lower than the loss of the reorganization,but no significant difference(P＞0.05);swimming group, treadmill group was significantly higher than that of freedom of the resumption of group(P＜0.05).Cortical bone mineral apposition rate(MAR),missing more than a quiet restructuring group(P＞0.05);the freedom and the restoration of swimming group compared with the trend of lowering the loss of the reorganization;treadmill group was significantly higher than the loss of recombinant(P＞0.05);treadmill group compared with the freedom to resume significantincrease(P＜0.01)Bone formation rate BFR/BS(%),higher than the control group lost recombinant (P＞0.05);the freedom of the restoration group was significantly lower than the loss of the reorganization,swimming group,treadmill group compared with the trend of lowering the loss of reorganization.Bone formation rate BFR/BV(%),loss of the reorganization was significantly higher than control group(P＞0.01);treadmill group was significantly lower than the loss of recombinant(P＜0.05);swimming group was significantly higher than the freedom of the resumption of group(P＜0.05).Osteoclast surface absorption(ORS),the freedom to restore group,swimming group reorganization of the relative loss of a downward trend;treadmill group was significantly lower than the loss of recombinant(P＜0.05);Swimming Group,the Running group and the freedom to run the restoration group,nosignificant difference.Conclusion:(1) In this experiment the body weight of rats in each group are increasing with aging. Suspension disposal methods can significantly inhibit the growth of body weight. Swimming exercise group observed in the vertical growth of the relative freedom of body weight recovery and treadmill Small;Help Desk movement running than swimming for the growth of body weight in rats is a suitable form of movement.(2) Allows suspension of bone formation activity,bone mass,bone structure,such as lower performance.(3) Different forms of exercise on osteoporosis to restore the role of rats significantly different results.Four weeks of exercise training on the solution of tail-suspended rats lumbar and femoral bone mineral density,bone metabolism and blood parameters tibia histomorphometry have clear targets,but better than the treadmill group swim team.(4) Different forms of movement for the role of bone metabolism are obvious differences between sites.(5) Bone mineral density and indexes have a significant correlation between bone mineral density and biochemical markers of bone metabolism and alkaline phosphatase and the ratio of acid phosphatase was significant correlation between... |
PDF Full Text Request