Synthesis And Characterization Of New Type Bifunctional Chelate Intermediates

Time-resolved fluoroimmunoassay is a new immunoassay technology established in 1980s,in addition to analysis with high sensitivity,wide range,good precision,and other advantages,in the immunoassay have more unique advantages.Through the use of rare earth complexes as a marker,by the method of measuring time delay,can effectively eliminate the interference of background fluorescence.The synthesis of bifunctional chelator was the key of time-resolved fluorescence immunoassay.The chelator should not only have the bifunctional properties:conjuncted with protein and chelated with rare-earth ion,but also transfer the energy to rare-earth ion.An ideal chelator is the key technology of DSLFIAIn this paper,tetra(ethyl) N,N,N'N'-[2.6-bis(3-aminomethyl-pyrazol-1-yl)-4-(4-nitro phenylethynyl)pyridine]tetrakis(acetate) and several important intermediates were designed, prepared and characterized according to correlative literature with the aim to satisfied the need of bifuncional chelatores for direct solid lanthanide time-resolved fluorescence immunoassay(DSLTRF1A).These intermediates were prepared from 2.6-dibromopyridine by oxidation,nitration,reduction,nucleophilic substitution,diazotization- Sandmeyer and NBS reactions and so on.During the experimentation,the structure of the products were determined by DSC,IR,~1H-NMR,MS,element analysis and so on to prove the reliability of these structures and svnthetic methods.