Research On The Correlation Between Predisposing Genes-UGTIAI TATA Box Mutation Between Hyperbilirubinemia And Dyskinetic Cerebral Palsy

Objective To study the correlation between predisposing genes—UGTIAI TATA box mutation between hyperbilirubinemia and .dyskinetic cerebral palsy so as to explore better theoretical foundation for the treatment of children with dyskinetic cerebral palsy.Methods 1. The subjects were divided into Group A which consisted of 41 cases of dyskinetic cerebral palsy; Group B which consisted of 38 cases of hyperbilirubinemia and Group C, a control group, which consisted of 36 healthy new-borns. 2. Peripheral vein blood samples were drawn from the new-borns of each of the groups, from which genomic DNA was extracted respectively according to kit instructions. 3.Polymerase chain reaction (PCR) was employed to amplify UGTIAI gene. TATA box, the UGTIAI gene promoter, primer sequence were designed. The product fragment length of 528bp was projected as well. 4. Relevant mutational sites were found after directly sequencing the purified PCR product.Results 1. There was no significant difference in gestational age, birth weight, gender difference and feeding patterns among Group A, B and C (P>0.05); 2. The genotype distributions among Group A, B, and C varies (P<0.03). heterozygote(simplified (TA)6/7) type gene mutation accounts for 36.6% of the all 41 cases in Group A while ((TA)6/7) type gene mutation accounts for 15.8% of the all 38 cases in Group B, 0% in GroupC .Moreover, homozygote(simplified (TA)7/7 )type gene mutation accounts for 2.4% in Group A and 0% in Group B,0% in GroupC respectively. The gene mutation rates in both Group A and B are higher than that in the control group and that in Group A is higher than that in Group B. Conclusion There are closely correlated. In Predisposing genes-UGTIAI TATA box mutation between hyperbilirubinemia and dyskinetic cerebral palsy...