Effects Of TSA On The Biological Behaviour And Expression Of RASSF1A Gene In Human Gastric Carcinoma Cell Lines

Objectives: To study the role of human gastric cancer cell lines SGC-7901 and MKN-28 were cultured in RPMI1640 and treated with different concentrations of TSA for different time, then many inspections and measurements have been done to illuminate the effect of TSA on the biological behaviour and expression of RASSF1A gene in human gastric carcinoma cell lines.Methods: Human gastric cancer cell lines SGC-7901 and MKN-28 were cultured in RPMI1640 and treated with different concentrations of TSA for different time. The proliferation and apoptosis of the cells was detected by MTT assay and flow cytometry, respectively. The morphocytology was observed by inverted phase contrast microscope. RT-PCR, Western blotting was applied to detect the RASSF1A mRNA and protein expression levels, respectively.Results: Inverted phase contrast microscope showed that SGC-7901 and MKN-28 cells without treated with by TSA were polygon or fusiform, arranged closely, even overlapped each other. But after incubated with TSA, cells became shrinkage and turned round, arranged loosely and monolayerly, adhered not good gradually, at last majority cells were floated in the nutrient medium.MTT assay demonstrated that T??isplayed a growth inhibitory effect on SGC-7901 and MKN-28 cells in a dose- and time- dependent manner after exposure to TSA at different concentrations and time. Flow cytometry analysis showed that the apoptosis rate in SGC-7901 cells went up significantly in a dose- and time- dependent manner after exposure to TSA at different concentrations and time and the cell cycle in MKN-28 cell was changed obviously, the number of G0/G1 stage cells was increased and the number of S stage cells was decreased. The results of RT-PCR and Western blotting demonstrated that RASSF1A mRNA transcription and protein expression were reactivated by TSA in gastric cells SGC-7901 and MKN-28 that not expressing RASSF1A . Conclusions: TSA can inhibit the proliferation and induce the apoptosis of gastric carcinoma cell lines by eliminating the aberrant histone acetylation status of RASSF1A gene.