Whole Lung Lavage At Different Stages Of Pulmonary Fibrosis In Experimental Rabbit's Blood Gas And Pathological Effects Of Intervention

Purpose:To observe the different stages of the whole lung lavage experimental pulmonary fibrosis of the rabbit blood gas and pathological effects of intervention. In order to understand the whole lung lavage treatment efficacy and timing of irrigation treatment, clinical treatment for pulmonary fibrosis patients to provide theoretical guidance, such as clinical application will be able to get good economic returns and social benefits.Object and Method: A randomized controlled experimen- tal study design. To the health of 34 New Zealand white rabbits were randomly divided into control group, 5, 5 model group and experimental group 21 (7d divided into groups, 14d group, 21d group 7). The first day of the experiment, each group in the ear artery blood testing blood gas, the trachea in the control group 2ml normal saline infusion, the model group and experimental group carried out a one-time infusion Pingyangmycin model to copy, then 7d group, 14d group, 21d in the first group 7d, 14d, 21d line of whole lung lavage. In the sixth week after the reunification of the ear artery blood contrast in each group were killed and pathological differences in blood gas.Results:1, the arterial partial pressure of oxygen in the experimental group before the start of the non- dis criminatory. In the fifth week, the model group (69.9±5.4), 14d group (76.2±6.4), 21 group (70.9±4.4) significantly (P <0.01) lower than the control group (96.5±7.9); 7d group (85.2±6.7 ) Significantly (P <0.05) lower than the control group, significantly (P <0.01) higher than the model group. PH, PCO2, SO2% in each group had no significant difference between.2, pre-test results of the light microscope: the first 14 days shows that a small number of congestive pulmonary edema, most of the alveolar wall thickening, thickening of the alveolar walls within Duoliang see a small number of neutrophils and lymphocytes, a little histiocytosis. Most see the first 28 days of normal lung tissue structure of the disappearance of alveolar air to expand, collapse, distort the bronchial expansion, marked proliferation of fibrous tissue, during which a small cell and tissue cells. 3, the sixth weekend pathology groups: control group: 5 rabbits with lung fibrosis alveolitis and no (0); model group: 5 rabbit lung tissue with severe pulmonary fibrosis, involved an area of 70% of the whole lung (Ⅲlevel); 7d: 2 rabbits with mild pulmonary fibrosis, and involved the whole area of the lung 15% (Ⅰlevel), 5 rabbits moderate pulmonary fibrosis, lung involvement of the entire area of 30% (Ⅱlevel) ; 14d group: 3 to moderate pulmonary fibrosis lung tissue, lung involvement of the total area of 45% (Ⅱlevel), 4 severe pulmonary fibrosis, the involvement of the whole area of the lung 55% (Ⅲ) level; 21d: 7 Lung tissue with severe pulmonary fibrosis, the involvement of the whole area of the lung 65% (Ⅲlevel). Model group and control group has a very significant difference (P <0.01), 7d group and model group significant difference (P <0.05), 14d group, 21d group compared with the model group was not significant (P> 0.05).Conclusion:After intratracheal injection of bleomycin can be a successful model of pulmonary fibrosis copy, whole lung lavage against experimental pulmonary fibrosis in rabbits PH value, PCO2, SO2% no significant change, early lavage can significantly prevent the arterial blood PO2 drepressing, and may reduce pulmonary fibrosis to some extent, delay the progress of the disease.