The Expression Of SNX3 In Postsynaptic Density Of Temporal Lobe Epilepsy Model

Objective:To investigate the expression of Sorting nexin 3 (SNX3) in PSD of temporal lobe epilepsy (TLE) model, so as to provide new cue for further studying the relationship of SNX3 and epileptogenesis of TLE.Methods:In this study, the Sprague-Dawley rats were randomly enrolled in Normal group (Norm) and experiment group. The model of TLE was induced by lithium-pilocarpine in the experiment group. And they were further divided into TLE group and Non-TLE group based on recurrent spontaneous seizure or not in the duration of 30 days after status epilepticus. PSD fractions were gradually obtained using sucrose gradient centrifugation. The expression of SNX3 was detected in the PSD fractions by western blot. Subcellular localization of the SNX3 in CA1 of hippocampus from three groups was tested using pre-embedding immunogold electron microscopy respectively.Results:1,The model of TLE: Status epilepticus were induced with the achievement ratio of 91.4%. The number of TLE rats was 32 (45.7%), and number of Non-TLE rats was 18 (25.7%) . The mortality rate was 20%.2,The analysis of PSD purity: Two markers of postsynaptic molecules NMDA receptor 1 and PSD-95 were expressed in P2, synaptosome and PSD fractions, but presynaptic marker synaptophysin was not expressed in PSD.3,The semi-quantitative analysis of the expression of SNX3 in PSD: Western blot results showed that SNX3 was detected positively in PSD fractions from three groups. Compared with the expressive level of SNX3 in the Non-TLE and Norm group, that in the TLE group obviously decreased (P<0.05) .4,The expression of SNX3 in PSD of CA1: Immunogold electron microscopy results showed that SNX3 gold particles existed in the PSD of CA1 in three groups. Compared with the number of gold particles in Norm group, those in the TLE and Non-TLE group decreased significantly (P< 0.05) . Compared with the number of gold particles in Non-TLE group, that in the TLE group decreased significantly, too (P<0.05 ) .Conclusion:SNX3 of PSD might participate in epileptogenesis of TLE.