Expression And Role Of CXCR3 In Patients With Psoriasis Vulgaris

ObjectiveDetect the expression level of chemokine receptor CXCR3 protein and mRNA in skin lesions and peripheral blood mononuclear cell (PBMC) of patients with psoriasis vulgaris and explore the role of CXCR3 in the pathogenesis of psoriasis vulgaris.MethodsTwenty cases of patients with psoriasis vulgaris and ten cases of healthy persons were selected.(1) Detect the expression of CXCR3 protein in skin lesions of patients with psoriasis vulgaris and normal skin tissues by immunohistochemistry method; (2) Determine the expression of CXCR3 mRNA in PBMC from patients with psoriasis vulgaris and normal controls by Reverse transcriptase polymerase chain reaction (RT-PCR) technique;(3) Analyze the relationship between the expression level of CXCR3 in skin lesions and PBMC of pateints with psoriasis vulgaris and PASI (Psoriasis Area and Severity Index).ResultsCXCR3 Protein was found to expressed in Keratinocytes (epidermis) and infiltrated lymphocytes (dermis) ,located mainly in cell membranes and cell plasma;No CXCR3 protein expression was found in the control group. The relative mRNA expression of CXCR3 in skin lesions of patients with psoriasis vulgaris was (1.44±0.72) ,in normal control it was (0.51±0.10); The relative mRNA expression of CXCR3 in PBMC of patients with psoriasis vulgaris was (1.49±0.10) ,while in control it was (0.45±0.09) . CXCR3 expression level in psoriasis vulgaris was obviously higher than that in control by statistical analysis,there was a significant difference between the two groups (P<0.05) .The expression level of CXCR3 in patients with psoriasis vulgaris was positively correlated with PASI.ConclusionChemokine receptor CXCR3 maybe involved in the pathogenesis of psoriasis vulgaris.