The Study On Transfection To Nasopharyngeal Carcinoma Cells By Hydroxyapatite-p53 Complex

Objective:To investigate the result of transfection to nasopharyngeal carcinoma cells by Hydroxyapatite-p53 complex.Method:Transmission electron microscopy of the hydroxyapatite nanoparticles by Polyethylenimine(PEI)(PEI-HAT) was performed,and their suspension stability observed under the room temperature,and for their biocompatibility with nasopharyngeal carcinoma cells CNE-1 cells (cell colony experiment),for the cytotoxicity of Polyethylenimine (PEI).PEI-HAT were observed and determined in condition of different concentrations for their capacity of DNA binding and transfection efficiency.Results:we have observed PEI-HAT is well dispersed and slightly agglomerated under TEM.PEI-HAT has good suspension stability under the room temperature.It showed significant cytotoxicity when used in the concentration of higher than 500μg/ml.We have found that not obvious cytotoxicity of PEI-HAT was revealed in the concentration of lower than 200μg/ml,the cytotoxicity is slowly down following concentration to descent.About the cytotoxicity of Polyethylenimine(PEI),we have found it showed significant cytotoxicity when used in the concentration of higher than 10μg/ml.the lowest cytotoxicity of PEI was revealed in the concentration of 5μg/ml.Agarose Gel electrophoresis retardation of PEI-HAT showed a good capacity of DNA binding in a wide range of concentrations.The Hydroxyapatite-p53 in the concentration of 175μg/ml,200μg/ml,250μg/ml,300μg/ml was available transfection,which was about 2%in nasopharyngeal carcinoma cells(CNE-1 strain).The transfection efficiency of liposome as the control group was approximately 60%in nasopharyngeal carcinoma cells(CNE-1 strain).Conclusion:PEI-HAT is well dispersed.it has good suspension stability under the room temperature.PEI- HAT showed a good capacity of DNA binding in a wide range of concentrations,and showed cytotoxicity to CNE-1 cells.Hydroxyapatite-p53 complex slightly agglomerated and the transfection efficiency of HAT-P53 complex in CNE-1 cells was Very lower than that of liposomes,In future we will further deeply study and improved the transfection efficiency.