| Objective:To explore the significance of the serum T lymphocyte subset and concentration of macrophage migration inhibitory factor(MIF) in the clinical diagnosis of autoimmune cause of POF and provide a basis for the etiological treatment.Methods:To detected 48 POF patients discharged chromosomal abnormalities and 20 cases of control group with antiovarian antibodies(AOAb),anti-distrand-DNA antibody(ds-DNA),antinuclear antibody(ANA) by immune gold-labeling dot detecting,and thyroglobulin antibody(TGA) by chemical fluorescence method,MIF and anti-zona pellucida antibodies(AZpAb) by ELISA,T-lymphocyte subsets by Flow cytometry,observing the positive ration and comparing the statistical differency of them,then according the results of AOAb, ds-DNA,ANA,TGA,AZpAb,to divided them into two groups as A group with one in five positive result or more and B group with all negative results,we analyzed statistically the results of T lymphocyte subgroup,MIF between the A group and B group. Results:1.The positive ration of ANA was 33.33%,Ds-DNA was 22.92%, AOAb was 18.75%,TGA was 31.25%,AZpAb was 27%,MIF was 29%, ANA TGA AZpAb and MIF were significantly higher than control group.2.There was no statistical significance in CD3,between experimental group which was 69.25±7.71%and control group which was 68.70±3.11%,but the CD4/CD8 in experimental group which was 1.72±0.78 was significantly higher than control group.Which was 1.09±0.20.The T-lymphocyte subsets is no statistical significance between A group and B group.(P＜0.05)3.Concentration of MIF in A group which was 127.70±63.34pg/ml was higher than B group which was 92.0±24.20pg/ml.(P＜0.05).Conclusions:1.The application of T-lymphocyte subsets detected by flow optometry to evaluate the immune etiological factor of POF in clinic has a limited value.2.The high level concentration of MIF could reflect the state of immunity of patients with POF in some extent,and could be the index of observing the development of POF.
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