Study On Dentification Ability Of Inbred Strain Mice Tooth Germs Implanted Into Oral Submucosa And Alveolar Process

Objective: To find a suitable environment for the bioengineered teeth in vivo and to investigate the influence of oral environment on the teeth development by observing the dentification ability of BALB/C filial mice tooth germs implanted into BALB/C male mice's oral submucosa and alveolar process.Method: The first molar tooth germs of the 4 days after birth BALB/C mice were implanted into oral submucosa and alveolar process one by one, and then extract them after 1,2,3 and 6 weeks regularly for histological observation.Result:1. The tooth germs in oral submucosa were growing well with continuing developing enamelum and pulpodentinal complex, and the dentinal tubules were clear. Although the dentine could extend toward the direction of the tooth, the tooth couldn't develop to be normal form.2. In alveolar process, only the first week implant was made into slice and there was development of dentinum, but the dental pulp was lost, the others were failed to be extracted. Conclusion:1. The environment of the BALB/C male mice's oral submucosa is favorable for the growth of inbred strain BALB/C mice tooth germs, which can provide a new environment for the bioengineered teeth development in vivo.2. The BALB/C mice tooth germs were developing to some extent when they were implanted into allograft BALB/C male mice's alveolar process, it needs a further development to be a scientific model.