The Effect And Mechanism Of Panax Notoginseng Saponins On The Focal Cerebral Ischemia-Reperfusion Injury In Rats

Objective:To study the protective effect and mechanism of Panax Notoginseng Saponins on the focal cerebral ischemia-reperfusion(I/R) injury in rats.Methods:Cerebral ischemia-reperfusion model in rats was induced by the middle cerebral artery occlusion(MCAO) method.Rats were divided randomly into sham operation group,model group,PNS treating group (25mg/kg) and Nimodipine(1mg/kg) treating group.Drugs were administered respectively 5min before cerebral ischemia,and 12h,24h and 36h after cerebral ischemia.Sham operation group and model group were given equal volume normal saline.The expression of protein GFAP,bFGF,NGF,Caspase-1,Caspase-3 and Caspase-8 were observed by immunochemistry after cerebral ischemia for 2 hours followed by reperfusion for 46 hours.Result:1.GFAP positive reactions increased obviously after cerebral ischemia-reperfusion.PNS could remarkably decrease the expression of GFAP in hippocampus(CA1)(p＜0.05).2.After cerebral ischemia for 2 hours followed by reperfusion for 46 hours,PNS could increase the expression of bFGF in medulla(P＜0.01).3.PNS could increase the expression of NGF(P＜0.05) after cerebral ischemia-reperfusion.4.The expression of caspase-1,caspase-3 protein increased obviously after cerebral ischemia-reperfusion,PNS could decrease the expression of Caspase-1 (p＜0.05)and Caspase-3(p＜0.01) compared with model group.5.PNS had no evident effect on the expression of caspase-8 after cerebral ischemia-reperfusion.Conclusion:PNS has a notable protection on cerebral ischemia-reperfusion damage.Its protective mechanism may be related with inhibiting the expression of GFAP,Caspase-1 and Caspase-3 and promoting the expression of bFGF and NGF.