Pinellia Heart-Purging Decoction And Dismantle Square To Infect To Pylorus Spiral Shell Bacillus Little Mouse Morphology, Stomach Of Mucous Membrane Study

AIM:Probe into tuber of Pinellia Heart-Purging Decoction and dismantle square to infect little mouse stomach influence and function mechanism,mucous membrane of morphology to pylorus spiral shell bacillus,and the whole side dismantle square group infect little mouse repair function that stomach organizes to pylorus spiral bacillus each, offer the experiment basis for the fact that prevent and cure the infectious stomach trouble of pylorus spiral bacillus clinically。Method:Divide the little mouse of 120 C57BLs into 10 groups at random:ⅠPinellia Heart-Purging Decoction all square group,Ⅱtuber of pinellia group,Ⅲsweet warm group,Ⅳbitter cold group,Ⅴtuber of pinellia add bitter cold group,Ⅵtuber of pinellia add sweet warm group,Ⅶbitter cold add sweet warm group,Ⅷpositive medicine contrast group,Ⅸmodel group,Ⅹnormal group.12 little mice respectively of every group,the male and female are half and half.Give the little mouse clear intestines to disinfect with the ammonia and benzyl and west forest in 4 days in succession first,begin to make the mould on the 5th day:Ⅰgroup irritate stomach pylorus spiral shell bacillus fungus liquid 0.2ml toⅨgroup each little mouse,irritate stomach fasting prohibitted water, irritate the stomach once every other day,amounts to 8 times.The end time irritate stomach build one day such as mould,collect four little mouse carry on stomach mucous membrane bacterium train with the urea enzyme testing at random.Result:Bacterium train,leather blue one dye mirror is it find G-curved bacillus to examine,fungus urea enzyme tests the masculine gender,is it make mould begin medicine irritate the stomach after succeeding to verify.Ⅰgroup offer equivalent traditional Chinese medicine of dosage irritate the stomach toⅦgroup according to each little mouse weight;Ⅷgroup irritate stomach equivalent ammonia benzyl penicillin of dosage,dilute with the distilled water into the necessary density.Model group and normal group each little mouse irritate distilled water,stomach of 0.2ml.Irritate the stomach for 8 days in succession,1 times a day,prohibit water fasting for 6 hours before irritating medicines.Irritate after the stomach,animal fasting can't help 12 hours such as ink,the heart collects blood and puts animals to death next day,it is and the intact it fetch stomach,it observe pathology shape where stomach organize change and measure stomach the cells not to wither dying and T lymphocyte groups of CD4~+and CD8~+expression.Result:1.Pinellia Heart-Purging Decoction square group,positive medicine contrast most of groups meet the obvious inflammation cell soak into all,compared with normal group this two groups do not have a significance difference(P＞0.05).Bitter cold add sweet warm group, bitter cold group and bitter cold add tuber of pinellia group can see a small amount of inflammation cell soak into,involve 1/3 of the stomach mucous membrane more.Model group can see a large number of inflammation cell soak into,involve 2/3,stomach of mucous membrane even the whole layer.Treat group square group,positive medicine contrast group,bitter cold add sweet warm group and model group is it have significance difference to compare with all(P＜0.01).2.Model group is it die cell increase,can is it is it lose the little body to wither to observe under the electric mirror to wither.Medication group square group,positive medicine contrast group,bitter cold add sweet warm group,bitter cold group and bitter cold add tuber of pinellia group compare with model group all,can reduce the stomach mucous membrane cell and wither and die,relatively has a significance difference(P＜0.01)effectively.3.The model group CD4~+is obviously reducing,CD8~+not obviously it increase,CD4~+/drop CD8~+ the proportion.Every group CD4 ~+expression to last group＞All square groups＞The bitter cold adds the sweet warm group＞Bitter cold group＞ The tuber of pinellia adds the bitter cold group＞The tuber of pinellia adds the sweet warm group＞Sweet warm group＞Tuber of pinellia group＞Model group.Every group CD8 express competence from high to successively a model group＞Tuber of pinellia group＞The tuber of pinellia adds the sweet warm group＞Sweet warm group＞Bitter cold group＞The tuber of pinellia adds the bitter cold group＞The bitter cold adds the sweet warm group＞All square groups＞The positive medicine contrasting and organizing＞The normal group medicine treats the group. All square groups among them,the positive medicine is contrasted and organized,the bitter cold adds the sweet warm group,last group at stomach mucous membrane cell CD4 ~+ the and CD8 ~+bitter cold group and bitter cold expression competence at and CD4 ~+/CD8~+ the proportion have significance difference as model group(P＜0.01).Conclusion:1.Pylorus spiral shell bacillus is it can cause stomach to be mucous membrane damaged to infect,cell is it is it increase with T cell inferior group lacking proper care to die to wither.Suppressing the pylorus spiral shell's bacillus activation,it is to treat the important link of infecting the relevant diseases of mucous membrane of stomach of spiral shell's bacillus of pylorus to dispel perverse trend.2.Pinellia Heart-Purging Decoction infect to pylorus spiral shell bacillus little mouse stomach mucous membrane have better repair function.Mainly it embody in it rush down tuber of pinellia there aren't heart the soups,suppress cell not to wither not dying and not regulating it CD4 ~+and CD8 ~+ expression.3.Pinellia Heart-Purging Decoction all square group with dismantle square group compare,square group all obviously superior to each dismantling the square group to pylorus spiral shell repair function that bacillus is infect each,rush down heart soup fever and chills of reason of it and tuber of pinellia and with,the more hard to turn on more bitter to lower,compatibility not precise and appropriate not strengthen body resistance and eliminating evil closely related with. Dismantle square group in order to strengthen body resistance and eliminating evil each and concurrently bitter cold that construct add sweet warm curative effect best group.Synthesize every index measure result, every medicine group infect to pylorus spiral shell bacillus repair function,stomach of mucous membrane from heavy to light to contrast group＞Pinellia Heart-Purging Decoction in the all square groups of heart soup＞The bitter cold adds the sweet warm group＞Bitter cold group＞The tuber of pinellia adds the bitter cold group＞Sweet warm group＞The tuber of pinellia adds the sweet warm group＞Tuber of pinellia group.