The Establishment Of Rare Earth-medicine Coordination Compounds Fluorescence Probe Systems And Their Analytical Applications

Minocycline(MNC) is a semi-synthetic second-generation tetracycline.It has a spectrum of activity and mode of action similar to that of tetracycline hydrochloride,but it is more active against many species.Chondroitin sulfate(CS) is a type of sulfated glycosaminoglycan commonly present in the cartilaginous tissue.CS has produced much more attention because of its critical roles in various physiological processes,such as the anticoagulation of blood,the adjustment of cell adhesion,inflammation and immunifaction,the hindrance of angiogenesis,antioxidation etc.Adenosine disodium triphosphate(ATP) is a type of coenzyme,it has the contribution to improve the metabolism of organism,to participate the metabolism of the grease,protein,saccharide,nucleinic acid and nucleotide in vivo.It is a main resource of energy in vivo either.As an exogenous drug,ATP can be employed to cure amyotrophy in progress,lingering effects of the cerebral hemorrhage,cardia insufficiency, myocardium affection and hepatitis etc.Luteolin(LU) is a representational and natural flavone,its distribution is very extensive in the vegetable kingdom.As a natural drug,luteolin can relieve a cough,make expectoration easy,and has the functions of antioxidant,antivirus,inhibition of impairment of endothelium and protection of neurons etc.In this thesis,the interaction of MNC,CS,ATP and LU with rare earth ions Eu³⁺ and Tb³⁺ are studied.It is found that they can form rare earth coordination compounds with rare earth ions under the optimum conditions.We have a successful determination of the four drugs based on the fluorescence of the coordination compound.In the first chapter,we summarize the luminescence mechanisms and the research progresses of the rare earth ions fluorescence probe,especially to the analytical applications of rare earth ions medicine coordination compounds.In addition,the physiological and pharmacological actions of MNC,CS,ATP and LU were summarized.In the second chapter,the MNC-Eu³⁺-SDBS luminescence system is studied in detail.It is found that under the optimum conditions,SDBS formed a ternary complex with MNC-Eu³⁺ and significantly enhanced the luminescence intensity of Eu³⁺.The enhanced luminescence intensity showed a good linear relationship with the concentration of MNC over the range of 4.0×10^-7-1.0×10^-5 mol/L with a detection limit of 2.0×10^-8 mol/L.This method is rapid and sensitive,and has been successfully applied for the determination of MNC in capsules and human urine/serum samples.The luminescence mechanism is also studied by the ultraviolet spectrophotometry and the change of surface tension.In the third chapter,the MNC-Eu³⁺-CS luminescence system is studied in detail.It is found that CS can significantly enhance the luminescence intensity of Eu³⁺,Under the optimum conditions,the enhanced luminescence intensity showed a good linear relationship with the concentration of CS over the range of 0.05-5.0μg/mL with a detection limit of 0.01μg/mL.The new MNC-Eu³⁺-CS is set up and has been successfully applied for the determination of CS tablets and injections.The luminescence mechanism is also studied by the ultraviolet spectrophotometry and the resonance light scattering spectra.In the fourth chapter,the BLFX-Tb³⁺-ATP luminescence system is studied in detail. It is found that ATP can significantly enhance the luminescence intensity of Tb³⁺ at the wavelenth of 545 nm,and the enhanced luminescence intensity showed a good linear relationship with the concentration of ATP over the range of 2.0×10^-6 mol/L-3.0×10^-5 mol/L with a detection limit of 8.0×10^-7 mol/L.This method is rapid and sensitive,and has been successfully applied for the determination of ATP in the injections.The luminescence mechanism is also studied by the ultraviolet spectrophotometry and the resonance light scattering spectra.In the fifth chapter,the LU-Tb³⁺-EDTA luminescence system is studied in detail.It is found that LU can significantly enhance the luminescence intensity of Tb³⁺ at the wavelenth of 545 um in the presence of EDTA,and the enhanced luminescence intensity showed a good linear relationship with the concentration of LU over the range of 5.0×10^-9 mol/L-5.0×10^-6 mol/L with a detection limit of 1.0×10^-10mol/L.This method is rapid,sensitive and has been successfully applied for the determination of LU in the human urine/serum samples. The main characteristics of this thesis are as follows:1.The tetracyclines drug MNC,the quinolones drug BLFX and the flavonoids drug LU can form coordination compounds with rare earth ions,the luminescence property of the complexes were studied and four new luminescence probes were set up to the determination of MNC,CS,ATP and LU based the fluorescence characteristics.2.The mechanisms were studied in detail by the ultraviolet spectrophotometry, Change of surface tension and resonance light scattering spectra.3.The above researches have potential value of application in the analysis of rare earth ion and medicine,which provided a certain theory and experimental foundation to the related study.