Expression And Significance Of PTEN,Akt And NF-kappaB Signaling Pathway In Adenomyosis

Backgroud:Adenomyosis(AM) is the growth of endometrial glands and stroma in the uterine myometrium,which exhibits a histopathological and clinical character of malignant tumors by invasive growth.But it is a benign disease of the uterus.And we used to call it as the endometriosis interna.It was named first by Frank.In 1972,Bird et al described the histopathological and clinical character in detail.Recently It is a common disease of gynecology field now,and is paid attention to.The incidence of AM is increasing,and is between 10%and 60%.Its exact pathogenesis is unclear.The continual progress of basic study will increase the realization of the pathogenesis of the adenomyosis.It will be advantageous of improve the therapeutic program to aim directly at the etiological factor of the adenomysis,and provide the forceful theoretical support for the clinical therapy.Objective:The adenomyosis and its eutopic endometrium were used to explore the mechanism of the PI₃K/PTEN/Akt and PI₃K/PKB/NF-kappaB signaling pathway in adenomyosis through monitoring the expression of cytokines including PTEN,Akt,P-Akt and NF-κB in adenomyosis tissues,in eutopic endometrium and normal endometrium.Methods: Immunohistochemical and ISH was employed to compare the expression of PTEN,Akt,P-Akt and NF-κB among ectopic lesions within myometrium of adenomyosis group(30 cases),its eutopic endometrium(27 cases),and normal endometrium(20 cases).Results:1.The proteinic of PTEN,Akt,P-Akt and NF-κB exist in adenomyosis tissues,in eutopic endometrium and normal endometrium.And The mRNA of PTEN and NF-κB is the same.2.In the gland cell,the proteinic expression of Akt,P-Akt in ectopic lesion of adenomyosis group was higher than that of eutopic endometrium in adenomyosis group (P＜0.05) and significantly higher than that of normal endometrium(P＜0.01).And the expression of Akt,P-Akt in eutopic endometrium of adenomyosis group was higher than that of normal endometrium(P＜0.01).In the stroma cell,the proteinic expression of Akt,P-Akt in ectopic and eutopic lesion of adenomyosis group was significant higher than in normal endometrium,but the comparison of the two groups had not statistical significance.3.The PTEN proteinic and m RNA expression on ectopic lesion was the lowest in the three groups.The PTEN proteinic and m RNA expression in normal endometrium group was the highest in them.and in ectopic lesion of adenomyosis group was the lowest(P＜0.01).The PTEN proteinic and m RNA expression of eutopic endometrium of adenomysis group was between that of normal endometrium group and that of ectopic lesion of adenomysis group(P＜0.05,P＜0.01)).The expression of Akt,P-Akt and PTEN was negative correlation in gland cell(r=-0.546,P＜0.01).4.The NF-κB protein was expressed in cytoplasm and nucleus,it's expression was higher in the gland cell of ectopic lesion than in that of eutopic endometrium in adenomyosis group(P＜0.05) and also higher than in that of normal endometrium(P＜0.01).And the expression of NF-κB in the gland cell eutopic endometrium of adenomyosis group was higher than in that of normal endometrium(P＜0.05).The tendency of proteinic expression of NF-κB existed in the stroma cell,but the comparison of the groups had not statistical significance.The expression of P-Akt and NF-κB was positive correlation in gland cell(r=0.626,P＜0.01).Conclusion:There were the expression of ptotein and mRNA of PTEN,Akt,P-Akt and NF-κB in adenomyosis tissues,in eutopic endometrium and normal endometrium.We could infer that PI₃K/PTEN/Akt and PI₃K/PKB/NF-κB signaling pathway might play an important role in the pathogenesis and the development of adenomyosis.