Expression Of Ezrin And CD44v6 In The Extrahepatic Cholangiocarcinoma And Its Relationship With Cancerous Invasion, Metastasis And Prognosis

Objective To study the expression of Ezrin and CD44v6 in the progression of cholangiocarcinoma,and the relationship between the expression of them and invasion and metastasis.Methods The expression of Ezrin and CD44v6 in 33 cases of cholangiocarcinoma were examined with s-p immunohistochemical technique in cholangiocarcinoma(33 specimens),tumor-adjacent(27 specimens) and normal epithelium of bile duct(12 specimens).Result The positive rate of Ezrin expression in cholangiocarcinoma,tumor-adjacent and normal epithelium of bile duct was 54.5%(18/33),59.3%(16/27) and 66.7%(8/12),respectively(P >0.05):The positive rate of CD44v6 expression in cholangiocarcinoma,tumor-adjacent and normal epithelium of bile duct was 27.1%(9/33),11.1% (3/27) and 0% (0/12),respectively(P <0.05). 2. In 33 cases of cholangiocarcinoma,The positive expression of Ezrin was related to size of tumor and methods of operations (P <0.05),but was not related to sex,age of patients, invasion,metastasis and TNM stage (P >0.05); The positive expression of CD44v6 was not related to sex , age of patients, TNM stage ,invasion , metastasis,methods of operations and size of tumor (P >0.05). There is no correlation between Ezrin and CD44v6. Multivariate analysis by Cox's proportional hazards model showed that only invasion and metastasis of lymphatic node is an independent prognostic factor of patients with cholangiocarcinoma.Conclusions The expression of Ezrin is not linked with formation,invasion, metastasis and prognosis,and is linked with growth of cholangiocarcinoma; The expression of CD44v6 is linked with formation of cholangiocarcinoma,and is not linked with invasion,metastasis and prognosis.