Effects And Mechanisms Of ATPR On Differentiation And Proliferation Of Human Lung Carcinoma Cell Lines

Objective: to investigate the effects and mechanisms of ATPR on differentiation and proliferation of adenocarcinoma and squamous-celled carcinoma of the human lung cell lines.( A549 and L78).Methods: Dividing A549 and L78 lung cancer cells into three groups. ATPR group, ATRA group and control group. In ATPR group ,cells were cultured with ATPR (0.01umol/L,0.1 umol/L ,1 umol/L,5 umol/L,10 umol/L) for 24h,48,72h,96h, The absorbance and growth inhibitory effect of ATPR on ( A549 and L78) was observed by MTT method. the cell cycle and was assayed by flow cytometry. cells were cultured with ATPR (1 umol/L,5 umol/L,10 umol/L) for 72h, HE coloretur and observe morphologic change of lung cancer cells. cells were cultured with ATPR with 1 umol/L for 72h, RT-PCR was used to analyze the expression changes of EGFR and RARα.Results : ATPR obviously inhibited the proliferation of (A549 and L78) cells ,and the cell cycle was arrested in G0/G1 phase.We observed the specificity EGFR and RARαstrap,through detecting A549 and L78 cells . The expression of EGFR in L78 cell of control group was on a high level .After incubation with ATPR,the expression of EGFR mRNA in L78 cell started to decrease after 24h;The expression of EGFR o in A549 cell was on a low level . the expression of EGFR mRNA in A549 cell was decreased in a time dependent manner. the expression of RARαin A549 and L78 cells were on a low level and the expressions were increased in a time dependent manner.Conclusion:1. ATPR minght inhibit proliferation and induce differentiation of (A549 and L78) .2. HE coloretur and observe morphologic change of lung cancer cells.3. EGFR and RARαmRNA were detected in A549 and L78 cells.4. ATPR upregulated RARαmRNA expression and down-regulated EGFR mRNA expression in a time dependent manner.