Novel Methods For Determination Of Hydroxyl Polycyclic Aromatic Hydrocarbons And Their Application

Polycyclic aromatic hydrocarbons (PAHs) which are ubiquitous persistent organic pollutants may induce lung and skin cancers in occupational settings. However, due to the complexity of exposure, urinary hydroxyl PAHs which are major metabolites of PAHs in human urine have been widely used as biomarkers of internal dose to evaluate the exposure to PAHs, especially 1-hydroxypyrene(1-OHP),α-naphthol andβ-naphthol. So, there is important practical significance to develop novel methods for determination ofα-naphthol,β-naphthol and 1-hydroxypyrene.In the chapter 2, a new method for simultaneous determination ofα-naphthol,β-naphthol and 1-hydroxypyrene has been established using synchronous fluorimetry technique. The measurement was carried out in sodium acetate-boroborax buffer solution (pH=5.0)withβ-cyclodextrin enhancing fluorescence at wavelength intervalsΔλ=23nm for 1-hydroxypyrene,β-naphthol andΔλ=175nm forα-naphthol, The linear relationship was obtained between the relative fluorescence intensity and concentration of 1-hydroxypyrene,α-naphthol andβ-naphthol in the range of 0.65-218.3μg/L, 3.6-1586.0μg/L and 2.8-1441.0μg/L, respectively. The limits of detection (LOD) for 1-hydroxypyrene,α-naphthol andβ-naphthol were 0.022μg/L, 1.53μg/L and 0.78μg/L with relative standard deviations (RSD) of 1.8%-1.6%, 2.3%-1.5% and 2.4%-1.9%(n=9), respectively. The proposed method showed sensitivity and reliability for the analysis of 1-hydroxypyrene,α-naphthol andβ-naphthol in urine samples with satisfactory results.In the chapter 3, we established a novel method for assayingα-naphthol,β-naphthol and 1-hydroxypyrene by RP-HPLC-UV. 1-OHP,α- andβ-naphthol were separated on DiamonsilTMC18(5um, 150×4.6nm)and detected at 280nm by using methanol-ammonium acetate buffer solution (70:30, v:v) as mobile phase when the column temperature was 35.0℃and the flow rate was 0.7mL/min.The calibration curves ofα-naphthol,β-naphthol and 1-OHP showed good linearities in the ranges of 0.3600-18.72μg/mL, 0.3600-18.72μg/mL, 0.2700-11.88μg/mL, respectively. The relative standard deviations (RSD) ofα-naphthol,β-naphthol and 1-OHP were 0.9%-1.1%, 1.3%-2.2%, 1.6%-3.2%(n=9)and the average recoveries were 102%, 103% and 99.0%, respectively. The proposed method was accurate and suitable for the identification and quantification of these compositions in human urine.In the chapter 4, a new detection method for 1-hydroxypyrene has been developed using a resonance light scattering (RLS) technique. In Tris-HCl buffer solution of pH6.5 and the presence of sodium dodecylbenzene sulfonate, the intensity of RLS was significantly enhanced due to the formation of ion-associate complex between brilliant green and 1-hydroxypyrene. The maximum RLS peak was located at 467.6nm. The enhanced intensity of RLS at this wavelength was directly propotional to the concentration of 1-OHP in the range of 4.3-1091.3μg/L(r=0.9994). The detection limit was 1.3μg/L, RSD=2.2%-5.8%, and the average recovery was 98.0%. The proposed method was used??detect 1-OHP in human urine and the results were satisfactory in comparison with those obtained by the method of high performance liquid chromatography.