Effect Of Jirjufushenning On Expression Of Osteopontin And CD68 Of Renal Tubule And Renal Protection In Diabetic Rats

Objective: To investigate effect of Jiejufushenning on expression of osteopontin(OPN) and CD68 of renal tubule and renal protection in diabetic rats.Methods : The diabetic SD rats induced by STZ were divided into four groups: diabetic control group, JJFSN group, Irbesartan group and JJFSN combined with Irbesartan group, and the normal control group. After experimental rats in every group were respectively treated for 12 weeks by above intervention methods. The relative kidney weight, fasting blood glucose, blood urea nitrogen, serum creatinine, and urinary albumin excretion rate (UAER) were detected by routine analysis methods. The pathological changes of the rat kidney were also observed by HE staining. Exprssion of osteopontin(OPN) and macrophages antigen (CD68) in renal tubule were determined by immunohistochemical technique, and OPN in renal was detected by Western blot methods.Results :Fasting blood glucose, UAER, blood urea nitrogen, serum creatinine, relative kidney weight were significantly increased, exprssion of OPN and CD68 were significantly increased, the tubular structure changed significantly under light microscope in diabetic control group. While the above indexes in JJFSN group, Irbesartan group and JJFSN combined with Irbesartan group were significantly lower than in diabetic control group (P<0.01), and abnormality of renal structure was improved, and the improvement of all indexes in JJFSN combined with Irbesartan group was most significant (P<0.01).Conclusion: Mechanism of protective effects of JJFSN on renal lesion may be related to decreasing the level of OPN in the renal tissue, and downregulating the expression of CD68.