The Research Of Constant Magnetic Field On Cobalt, Chromium Ions In Human Monocytes To Release TNF-a And Apoptosis

Objective:Discussion the constant magnetic field on the metal ions of cobalt, chromium in human peripheral blood mononuclear cells secrete tumor necrosis factor, and explore its dynamic changes of apoptosis and Caspase-3 activity, to search a promising therapeutic candidate for the prevention and treatment of aseptic loosening. Method:CoCl2 powder and CrCl3 powder were dissolved in the asepsis injecting water. Mononuclear cells from human peripheral blood, were taken and cultured with Co2+,Cr3+ ions in different magnetic field of 10Gs,100 Gs,1000Gs. Experimental sub-9 Group: control group,Co2+,Cr3+ group, Co2+,Cr3+, respectively, add to different magnetic field strength (10Gs, 100Gs, 1000Gs) group, Cultured cells in each group 12,24,48 hours supernatant using ELISA assay of tumor necrosis factor (TNF-α) content; Apoptosis was detected by TUNEL, the colorimetric assay of Caspase-3 activity.Result:After 12h by the magnetic field exposure, the magnetic field treatment group TNF-αin both lower than the non-magnetic treatment group (P<0.05), and a time-dependent intensity. 100Gs in dealing with the magnetic field and 1000Gs group, apoptosis was significantly higher (P<0.05), and with the increasing strength of the magnetic field, Caspase-3 activity gradually. The magnetic field 10Gs Group apoptosis and Caspase-3 activity had no significant effect of the change (P>0.05). Conclusion:Within a certain range of magnetic intensity , constant magnetic fields inhibit human mononuclear cell stimulated by Co2+andCr3+ ions to secrete TNF-αand promote their apoptosis.It provides a theoretical basis for the prevention and treatment of aseptic loosening.