| Objective:To study the effects of ketamine general anesthesia during gestationperiod on the learning and memory functions of Sprague-Dawley rat,s offspring.Method:Thirty pregnant Sprague-Dawley (SD) rats were randomly divided into ketamine group and Control group,fifteen in each group. Ketamine was injected intravenously to the rats in the ketmine group for 2 hours, While ketmine was replaced with eaqual volume physiological saline solution for the rats in the control group.The learning and memory functions of the offspring rats were tested by Morris water maze test at different postnatal time intervals,20 days and 30 days respectively.The morphological changes of hippocampus tissue were analyzed under electrical microscope for the offspring rats. At the same time, the expression of the immediate early genes, c-fos and c-jun, were checked by RT-PCR.Result:The latent period of the offspring rats in ketamine group was longer than that in the control group at 20 days and 30 days postnatly. The latent period of the second times of the offspring rats in ketamine group was significantly longer than that in the control group at 30 days postnatly (p<0.05).The hippocampus tissue of the offspring rat,s in the ketmine group was significantly damaged. The expression of the immediate early genes, c-fos and c-jun, appeared in both the offspring of the two groups and their gray value were equal.Conclusion:Ketmine general anesthesia during gestationperiod could cause learning and memory functional injury to the offspring rats.It is related to the neuronal damage in the hippocampal tissue caused by ketmine. |
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