The Changes Of Hippocampal Proteomics In Mesial Temporal Lobe Epilepsy

Research background Mesial temporal lobe epilepsy (MTLE) is the most common type of intractable epilepsy, about 60- 70% of patients with intractable focal epilepsy suffered from it.Hippocampal sclerosis is the symbolized pathologic finding in MTLE, which can be observed in approximately 65% of people with MTLE, while the pathogenesis of MTLE remains uncertain.The pathogenesis of MTLE have been found to associate with aberrant mossy fiber sprouting in the hippocampus, apoptosis and the abnormality of neurotransmitters and receptors. Proteomics technology is a new subject have been widely used to study the function, organization, diversity and dynamic variety of whole cell or even whole tissue simultaneously, which make it possible to study the pathogenesis of MTLE.Objects This experiment is to establish the 2-DE profile of hippocampus proteome of MTLE and normal through proteomic technique, and to sieve the disease-related proteins to provide clues for a deeper studying of the MTLE pathogenesis and offer target reference for the further therapy.Methods Hippocampus samples were obtained respectively from MTLE and normal patients. We separated proteins with immobile pH gradient (IPG) 2-DE technology and protein spots were visualized by Coomassie Brilliant Blue staining. The stained 2-DE gels were scanned on the Imagescanner and obtained picture through Lab-scan software. Also, analysis the images with PDQuest software and the different proteins spots were detected between the MELE and normal patients. The clearly discerned spots which are expressed only in MTLE hippocampus were chosen to carry out Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analysis to obtain the Peptide mass fingerprinting (PMF). Finally, we get the basic information of the proteins through bioinformatics to definite specific proteins.Result Mean protein spots of the 2-DE gels were 854±40 and 833±56 with MTLE and normal patients respectively. Fifty-four different protein spots were screened between the MTLE and normal group, of which 30 protein spots showed up-regulation in the MTLE group, 10 down-regulations in the MTLE group, and 11 sopts only occurred in normal group and 3 spots only occurred in MTLE group. MALDI-TOF-MS was used to analyze forty-one differential protein spots and 41 PMF were obtained. After searching the data base, 34 proteins were matched. We also sort these proteins by their function. They belong to cytoskeletal protein, heat shock protein, metabolic enzyme, peroxiredoxin, signal transduction protein and other functional proteins.Conclusion The 2-DE profile of hippocampus proteome was successfully established in MTLE and normal patients through proteomic technique. By the differentiated analysis of these hippocampal 2-DE gels, the differences of hippocamp proteome profiles, including numbers and densities, were discovered between MTLE and normal group. They may be closely related to the development and progression of MTLE.