Experimental Study Of Prefabricated Complex Tissue Flaps Containing Nerves And Vessels

Objective: Severe complex tissues injury is difficult to deal with, and its clinical treatment result is not satisfactory, especially when standard donor sites are not available or do not provide sufficient tissues. Prefabricated complex tissues flaps , as a new method, can be applied for treament of the defects of complex tissues. In the study we have succcessfully designed prefabricate complex tissues flaps containing vessels and nerves so as to study the feasibility of the prefabrieated flaps in treating severe trauma and try to improve its growth, so as to provide a method in treating severe trauma.Methods: Forty wistar rats were randomly divided into two groups , prefabricated complex tissues flaps were generated by dissociating the right femoral vascular, femoral nerve and sartorius, implanting them underneath the abdomal flaps. Fibrin glue with Basic fibroblast growth factor ( bFGF 10μg )was sandwiched under the skin in experimental group, while Fibrin glue without bFGF was sandwiched in control group. Two weeks later an abnominal island tissue flap based solely on the implanted tissue and vessels was elevated, the prefabrieated island flap was satured back , then it was evaluated a week later. Observing methods: 1. survival areas of prefabricated complex tissue flaps were observed by means of general investigation and its survival area rates were caculated .2 .Flap vessels were stained with basic violet 3 to observe blood circulation by means of general investigation and microscope. 3. Microvessel density of the prefabricated complex tissue flaps were observed by histological examination .Results: 1. There was none of severe infection and death overal. Two cases were failed because of the pedicle shedding. The other flaps had good blood circulation after they were raised. 2. There was a significant increase in survival area of prefabricated complex tissue flaps in experimental group compared with control group, significant differences were detected between groups (P<0.01) .3. The biodistribution studies of basic violet 3 revealed that the flaps were revascularized suffiently. The microvessel with basic violet 3 stained located in epidermis and dermis. 4. We found microvessel density and blood flow of the experimental group was better than the control group by means of immunohistochemistry stain, significant differences were detected between groups (P<0.01) .Conclusion: Through this study ,we have testified viability of refabricated complex tissues flaps on the model. In this study, it is revealed that the Basic fibroblast growth factor that was slowly released by fibrin glue can be used to improve the growth areas and the microvessel density of prefabricated complex tissues flap .As a new method, prefabricated complex tissues flaps can be applied for treament of the severe trauma, especially in the defects of complex tissues.