Analysis The Association Between Levels Of Platelet Activation Markers In Neonate Hyperbilirubinema, Myocardial Enzymes, And Other Relative Factors

OBJECTIVE: To investigate the relationship and clinical value of levels of platelet activation markers in patients with Neonatal Hyperbilirubinemia in perpheral blood and myocardial enzymes or other related factors.Methods: Levels of platelet activation markers in perpheral blood of 30 neonates with Hyperbilirubinemia were measured by flow cytometry (FCM), which made in American. Fifteen health newborns served as a control group. In the Morning, took 1ml blood in the femoral vein from the patients who was on empty stomach, 20g/LEDTA-2Na 0.1ml anticoagulant, shook gently to avoid excessive vibration of this step by hand operation. Dealt with the initial treatment In half an hour and detected by machine by One hour period . The two were in the test tube by adding CD41-FITC10μl, and then joined IgG1-PE and CD62P-PE reagent 10μl, with diluted liquid 200μl PBS, adding 5ul whole blood, dark room temperature for 15 minutes before the testing machine. Use of negative control tubes (CD41-FITC+IgG1-PE)to adjust voltage, the detection marker CD62P-PE. Myocardial enzymes and liver function were measured by automatic biochemistry analyzer.RESULT Analysis showed that levels of platelet activation markers in patients were significantly different when comparing with the normal control group(P <0.01). By multifarious factors linear regression analysis ,we drawn a conclusion that the rate of total bilirubin /albumin (B/A) had a positive correlation with the group of high levels of platelet activation markers(>50%), R=0.932. While total bilirubin had a positive correlation with the group of middle levels of platelet activation markers(<50%)R=0.536; levels of platelet activation markers only had a positive correlation with the group of myocardial enzymes isozyme(CK-MB). R=0.688.This shown that the relationship between Hyperbilirubinemia and organ damage, particularly in the heart and liver damage .CONCLUSION It had a significant difference between experimental group and the control group; levels of platelet activation markers may serve as a reference index when inflammation damage to human body organs was being evaluated. In the clinical treatment of Hyperbilirubinemia, it may provide a reference medicine in the use of anti-free radical and improving microcirculation medicines.