Studies On The Anti-Tumour Activity And Immunomodulatory Function Of Polysaccharide Of Carceps Sinensis (Csp)

Cordyceps sinensis, is a parasitic complex of fungus of and caterpillar, which was produced mainly in High-altitude areas of China, such as Oinghai, Tibet, Sichuan, Yunnan, Guizhou. It has been used widely in traditional Chinese medicines (TCM) for centuries. Cordyceps sinensis has wide functions on anti-tumor, anti-aging, lowering blood pressure and sugar, immune regulation, Recently, as a very active area of research, more and more attention have been concentrated on polysaccharide of Cordyceps sinensis(CSP) .Polysaccharides of Cordyceps sinensis has complex biological activities. Even though some researches have been done on its immunoregulation and antitumor activity, but is still a lack of systematic studies. In this research, CSP was extracted from fungus of Cordyceps sinensis through liquid culture, immunomodulation and antitumor activity of CSP were studied systematicly.Firstly, the imunomodulatory function of polysaccharide of Cordyceps sinensis (CSP) was studied on phagocytosis of phagocytes, proliferation of T and B lymphocytes, production of immunoglobulin. The results showed that CSP could promote the proliferations of T cells induced by anti-CD3 treatment and B cells induced by LPS treatment from mouse spleen cells, enhance the production of IgM induced by LPS treatment, but had no effect on the IgA production. CSP had different functions on the IgG subtypes, it could suppress IgG2a production obviously, but to IgG1 it was dosage depended. At the dosage of 5μg/ml, the IgG1 production was promoted (P<0.001), but when the dosage was up to 50μg/ml, CSP displayed the obvious inhibitory action. Then the anti-tumour activity of CSP was conducted in this research, the results showed that the growth of S180 tumour and proliferation of S180 cells were suppressed by CSP.