The Expression And Relationship Of ROCK-2 In The Breast Infitrating Ductal Carcinoma

OBJECTIVETo detect the expression of ROCK-2 proteinum, then explore whether it closely related to origin, development and transfer of the breast infitrating ductal carcinoma, also the relationship with other index of breast cancer.METHODWe collected 68 cases of the breast infitrating ductal carcinoma with pathologic diagnoses and detailed information (provided by the Department of Pathology, The 88th Center Hospital of PLA,2007,1~2008,5). In addition, choosed 5 cases as control. Detected the expression of ROCK-2,C-erbB-2,PR and ER in the breast infitrating ductal carcinoma and fibroadenomas with SABC immunohistochemistry, analyzed the data with pathology image analysis system (MIAS 3.0).RESULT1. The expression of ROCK-2 in the natural tissue and benigh tumor of breast is negative;2. The expression of ROCK-2 has significant differences (P<0.05) in each pathology grade(gradeI: 29.76±7.05; gradeII: 49.36±9.82; gradeIII: (68.33±7.81)and between the three grades(P<0.05), also the expression of ROCK-2 has positive relation with the pathology grade of breast cance;3. We divide the 68 cases into two groups according to lymphatic metastasis:40 cases accompanied by lymphatic metastasis; 28 cases have no lymphatic metastasis. The expression of ROCK-2 has significant difference (P<0.05)between the two groups, and the former is higher than the later;4. It also can be divided into two groups according to the expression of C-erbB-2 in all cases: 45 cases are positive expression; 23 cases are negative expression.The positive expression of ROCK-2 between the two groups has significant difference (P<0.05), and the former is highter than the later;5. We further divide the 68 cases into two groups according to the expression of ER:50 cases of positive expression;18 cases of negative expression.Between the two groups we found that the expression of ROCK-2 was not significant (P=0.204). The same method we also found that: the expression of ROCK-2 has no statistical significance between the negative and positive groups of PR expression (P=0.062).CONCLUSION1. The expression of ROCK-2 in the normal tissue and benigh tumor is negative;2. The expression of ROCK-2 in the breast infitrating ductal carcinoma has positive relation with pathology grade and lymphatic metastasis, suggested that Rho/ROCK signal transmit path may take an important advanced role in the origin, development and transfer of the breast infitrating ductal carcinoma;3. The expression of ROCK-2 has positive relation with the expression of C-erbB-2 in the breast infitrating ductal carcinoma, suggested that ROCK-2 and C-erbB-2 may take an important role in the invasion and transfer of the breast infitrating ductal carcinoma;4. In the breast infitrating ductal carcinoma, the expression of ROCK-2 has no obvious relation with the expression of ER and PR.