The Effect Of 5-Fu With CF On Apoptosis Of The Mammary Cancer Cells

5-Fluorouracil (Fluorouracil, 5-Fu) is a anti-tumor drug, which is in possession of speediness, high efficiency and low toxicity, and has been the first choose drug in malignant cancer therapies, e.g mammary cancer, gastro-intestinal tract cancer, liver cancer, and so on. At the mean time, it has been found that the effects of 5- Fluorouracil in different clinical therapies were different because of its toxicity, so that it is somewhat restricted in its applications of clinic. Along with the modern medicine biological technology development, 5-Fluorouracil associated with other medicine was used to enhance the drug efficacy successfully. However, some kinds, dose-effect and the mechanisms of 5- Fluorouracil associated with other drugs, are still unknown clearly.Some studies shows that reductive Leucovorin (CF) with low toxicity as assistant enzyme has an important role in DNA corporation and could enhance the effect of 5- Fluorouracil Presently, along with the develop of technology, some people use to carry on certain process to 5-Fluorouracil, or use other medicine together, seek the low poisonous highly effective way, overcome 5-Fluorouracil anticancer treatment medicine's some shortcomings, enable it to obtain a more widespread application in the clinical aspect. Thus, here we adopt the way of MTT, and so on, take 5-Fluorouracil with Calcium Leucovorin(CF) on MCF-7 cell line, which is used for different effect of the dose of 5-Fluorouracil Calcium Leucovorin, also to detect the best time and the dose of the drugs with MCF-7 cell line. After take doses of 5-Fluorouracil and Calcium Leucovorin, we observe the cell apoptosis with morphological ways, AO/EB staining, DNA ladder and also we use Cyto-Flows to see the change of phases with MCF-7 cell lines. Also, we detect the expression relative protein of Bcl-2, Bax and so on, discuss possible mechanisms, in order to offer some theories and gist of upgrade the effec of application in clinic, lower the toxicity.Our results showed that:(1)This topic that mechanism of restraining cancer in view of 5-Fluorouracil, find the best dose to MCF-7 cell line to with Fluorouracil and also the best response time; Different doses are used to see the different specificity and the best time and dose. There are differences according to the different cell logarithm period, which will cause the cell growth condition at the logarithmic growth time, to take each experiment smoothly. MTT method to find the best medication dosage as well as best medication time, with different dose of 5-Fluorouracil with Calcium Leucovorin (2.5μg/ml,5μg/ml). 5-Fluorouracil, which is used single, is not better than that both used 5-Fluorouracil and Calcium Leucovorin.(2)Using morphology examination and using fluorescence staining technique under the microscope, cells of apoptosis are getting round and round, the color of DNA become dark and some fell off. We see the changes of phases to the cells which are dosed with 5-Fluorouracil and Calcium Leucovorin, with cyto-flows, different period of apoptosis could be detected.In the early phase of apoptosis cells, we could see some small bodies in cells, late phase of apoptosis cells become red, shapes are similar to the early ones.The death cells are all red without intact shapes.(3)After dosing with 5-Fluorouracil and Calcium Leucovorin with some time, collecting cells, using relative antibodies, we detect the expression of apoptosis relative proteins bcl-2, Bax, and p53. The result is that the expression of Bcl-2 is falling, the expression of Bax is rising.Conclusion:(1) 5-Fluorouracil would get more effect on cancer cells if it is used together with Calcium Leucovorin. To some sides, there are some dose depending between the medicine and the ratio of death of MCF-7 and other cancer cells.(2) The cells rise with the doses of 5-Fluorouracil and Calcium Leucovorin, and both the phases of early and the late cells rise, too.(3) Apoptosis of cancer cells are related to both Bcl-2 and Bax, which the ratio of Bcl-2 and Bax are also taken some rising effect.