Changes In The Amount And Function Of The Early And Late Endothelial Progenitor Cells Colonies Derived From Peripheral Blood In Patients With Acute Myocardial Infarction

Objective To explore changes in the amount and function of the early and late endothelial progenitor cells colonies derived from peripheral blood in patients with acute myocardial infarction (AMI)Methods Peripheral venous blood was obtained from 28 patients with AMI,26 patients with stable angina pectoris (SA),and 20 non-patients with coronary heart disease (CHD).Mononuclear cells were isolated with density-gradient centrifugation and were cultured in EGM-2MV medium. Endothelial progenitor cells (EPCs) were identified by examining the morphology, double fluorescence staining cells and cell markers,and the early and late colonies were counted after 7 and 21 days respectively. The levels of stromal cell-derived factor-1 (SDF-1) in conditioned medium were detected with immunosorbent (ELISA) method. EPCs proliferation and migration were assayed by MTT assay and modified Boyden chamber assay respectively.EPCs adhesion assay was performed by replating those on fibronectin-coated dishes, then adherent cells were counted.Results The amount of the early colonies in AMI group was significantly higher than SA and non-patients with CHD groups (6.7±2.1 vs 3.0±1.1,6.7±2.1 vs 4.1±1.6,P<0.01),but the levels of SDF-1 secreted by the early colonies in AMI group were obviously lower than SA group (103.07±15.12 vs 119.38±24.03,P<0.05) and non-patients with CHD group (103.07±15.12 vs 142.75±29.91,P<0.01). The number of early colonies and the levels of SDF-1 in SA group were obviously lower than non-patients with CHD group (P<0.05,P<0.01). The amount and function of proliferation,migration and adhesion of late EPCs in AMI group were significantly lower than SA group (1.6±1.2 vs 2.6±1.5,0.294±0.051 vs 0.340±0.057,8.9±1.9 vs 10.4±2.2,19.7±4.9 vs 23.4±5.1, P<0.05) and non-patients with CHD group (1.6±1.2 vs 3.9±1.8,0.294±0.051 vs 0.477±0.085,8.9±1.9 vs 14.5±3.3,19.7±4.9 vs 31.0±6.7,P<0.01),and these index in SA group were also lower than non-patients with CHD group (P＜0.01)Conclusion Increased early colonies amount can be observed at the onset of AMI, but the secreting function of early colonies is severely impaired. Reduced late colonies amount and impaired proliferative, migratory and adhesive function of late EPCs can be observed. Reduced amount and impaired function of early and late EPCs can be observed.