Primary Valuation On The MicroRNA Difference In Carcinogenic Pleural Fluid And Benign Pleural Effusion For The Diagnosis Of Early Lung Cancer

Objective The aim is to explore the potential application of miRNAs as the diagnostic biomarkers to discriminate lung malignant effusions from benign ones.Methods To obtain a molecular signature for malignant effusions, microRNA microarray was employed to detect the existence of microRNAs and their levels in 5 plenral fliuid samples (2 benign/3 malignant). The microRNAs count signal data was analyzed by SAM software to select the miRNAs comparatively different between these two groups. Real-time PCR was applied to identify the different expression levels of these miRNAs in 18 pleural effusion samples (9 with benign/9 malignant), which included those 5 samples used in microRNA array.Result The existence of MicroRNAs were confirmed by both microRNA microarray and Real-time PCR technology. The expression level of MicroRNAs were different among 5 plenral fliuid samples. The SAM statistical result showed there were 36 MicroRNAs with more than 2.5 fold change. MiR-181c*,miR-374a*,miR-629* and miR-1227 were choosed to expand by Real-time PCR. The miR-1227 was down regulated obviously in the experiment group compared with the control group (P＜0.05).Conclusion MicroRNAs exist in plenral fliuid; the expression levels of miR-1227 in pleural effusions may be helpful in diagnosing malignant pleural effusions.