Effect Of Emodin And Its Derivatives Combined With Paclitaxel On The Growth Of Ovarian Cancer A2780 And A2780/TAXOL

Objective:(1) To investigate the effect of emodin and its three kinds of derivatives respectively on the growth of ovarian cancer A2780 cells and A2780/TAXOL. (2) To investigate the effect of emodin and its three kinds of derivatives respectively combined with paclitaxel on the growth of ovarian cancer A2780 and A2780/TAXOL.Methods:Ovarian cancer A2780 and A2780/TAXOL were cultured in vitro and then randomly divided into ten groups:control group (A group); emodin group (B group); emodin derivatives 1 group (C group); emodin derivatives 2 group (D group); emodin derivatives 3 group (E group); paclitaxel group (F group); paclitaxel combined with emodin group (G group); paclitaxel combined with emodin derivatives 1 group (H group);paclitaxel combined with emodin derivatives 2 group (I group); paclitaxel combined with emodin derivatives 3 group (J group). In order to form emodin's derivatives,we used semi-chemical synthesis method in emodin nucleus to introduce different groups.The given groups were intervened by emodin and its derivatives respectively combined with paclitaxel,.IC50and the cell growth inhibition were detected by MTT, cell cycle and apoptosis were detected by FCM.Results:1. Intervened by different concentrations of emodin and its three kinds of derivatives respectively, the growth of A2780 cells was inhibited. There was significant difference between either of two different concentrations (P＜0.05). With the increasing concentrations of emodin, the growth inhibition rate was increased. 2. Intervened by different concentrations of emodin and its three kinds of derivatives respectively, the growth of A2780/TAXOL cells was inhibited. There was significant difference between either of two different concentrations (P＜0.05).With the increasing concentrations of emodin, the growth inhibition rate was increased.3. Intervened by different concentrations of emodin and its three kinds of derivatives respectively, there was significant difference between either of two different concentrations (P＜0.05).With the increasing concentrations of emodin, the growth inhibition rate was increased.4. In the group intervened by paclitaxel respectively combined with emodin and its three kinds of derivatives, the growth inhibition rate of A2780/TAXOL cells was higher than that intervened by paclitaxel alone, or alone intervened by emodin and its three kinds of derivatives respectively.5. In the group intervened by paclitaxel respectively combined with emodin and its three kinds of derivatives, the apoptosis rate of A2780/TAXOL cells was higher than that intervened by paclitaxel alone,or alone intervened by emodin and its three kinds of derivatives respectively.6. The apoptosis rate of A2780/TAXOL cells was increased, intervened by paclitax respectively combined with emodin and its three kinds of derivatives.More cells were blocked in G phase, and the number of cells was significantly decreased in S phase.Conclusions:1. The growth of ovarian cancer A2780 and A2780/TAXOL can all respectively be inhibited by emodin and its three kinds of derivatives.2. Emodin and its three kinds of derivatives respectively combined with paclitaxel have synergistic effect on the growth of ovarian cancer A2780/TAXOL.3. The possible mechanisms of the synergistic effect of Emodin and its three kinds of derivatives respectively combined with paclitaxel:Ovarian cancer A2780/TAXOL's resistance can be reversed by Emodin and its three kinds of derivatives,which enhance the paclitaxel-induced cell apoptosis rate.