| Objective:To investigate the expression patterns of glial fibrillary acidic protein (GFAP) of astrocytes in brain and spinal cord after the Spinal cord hemisection injury of Rats.Methods:To set up the Spinal cord hemisection injury model of Rats. A total of thirty healthy adult SD rats whose body weights are between 180 to 220g. They were randomly divided into the following groups:the control group (n=6) and the experimental groups (n=24). Then the experimental groups were subdivided into four groups by the survival times after trauma:1h,2h,24h and 72h with each group consisted of six rats. Immunohistochemisty was used to examine the expression of GFAP and the quantitative and morphological changes of astrocytes, the adjacent sections were undergone the Nissl's staining to discriminate the locations of nuclei.Resulst:The immunoreactivity of GFAP in all parts of M1, S1, CA1, CA3, DG, Ventral thalamus (VG), Medulla oblongata and the injuried spinal cord of each experimental group were significantly enhanced compared with the control group, respectively (p<0.01). The proliferation of astrocytes which located in the place of injuried spinal cord was the most obvious and it was the same as the changes of astrocyte's configuration. The changes in configuration and number of GFAP-positive cells were relatively moderate in other observed parts. In the hippocampal CA3 region and the injuried spinal cord, the sample mean's differences of the reactive astrocytes were not statistically significant between the 24h group and 72h group (P>0.05). Beyond these, the differences of sample means among the groups in each observed parts were obvious and there were statistical meanings (p<0.01).Conclusion:The reactive astrocytes are occurred in proliferation early. In the corresponded regions, the shapes and numbers are changing. Over time, the proliferation rate will drop but the numers of astrocytes are still more than the ones in normal level. |
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