The Function Of ITIH4 Gene In Malignant Tumor Of Ovary

Epithelial ovarian cancer incidence of gynecological malignancy is accounted for a third, but the first rate in malignant disease. Due to the ovary hidden in the depths of the anatomical in early onset, pelvic lack of specificity symptoms and signs, it is difficult to diagnosis and early detection, about 60-70% of ovarian cancer patients diagnosed when already belongs to the terminal. For nearly 30 years, although experts worldwide gynecological tumors through unremitting efforts, and constantly improve operative, found newer and better chemotherapy drugs, adding new chemotherapy, but the 5-year survival rate hovers around 20% to 30%. So, looking for new diagnostic methods and new treatment method is currently ovarian cancer research. This study is the first in the group using surface-enhanced laser desorption ionization time-of-flight mass spectrometer (-)-SELDI TOF-and ELISA technology found MS Z (M/over the feet, or 3272 ITIH4) is secreted in oarian cancer patients serum segment is raised, as a kind of adhesion such tumor metastasis and exudate is closely related to the process, human alpha trypsin H4 heavy chain system (trypsin inhibitor of alpha-ITIH4 H4 in ovarian) proliferation, metastasis plays an important role. This topic through a series of molecular biology technology and experiment of cells in vitro ITIH4 in oarian cancer metastasis processes and mechanisms of the research, and obtained the following:1 for real-time PCR quantitative fluorescence in 49 cases,20 cases of oarian cancer ovarian benign tumor,16 normal oarian tissue ITIH4 organization mRNA quantitative analysis, ITIH4 in malignant ovarian tissue with good expression and normal tissue there difference (p< 0.05), in the expression of malignant tissue ITIH4 quantity below normal tissue, with no obvious difference in benign organization;2. through constructing human alpha trypsin H4 heavy chain system (trypsin inhibitor of alpha-ITIH4 H4 shRNA gene expression vector), ovarian cancer cell, its transfection of ITIH4 gene interference efficiency. Then screened stability of cell lines, a series of interference experiment, opposite ITIH4 verify functional gene functions, Cell growth curve shows interference group of cells, and a double time statistically significant (P= 0.001). Interference and control groups formed induction.blood respectively are 45.7±0.7% and 31.4 within±0.53, comparison, p= no statistical significance. In the experiment, the ability to move pGPU6-ITIH4-0.4481 is 0.0277±917, compared with negative groups, pGPU6-ITIH4-917 group migrating ability than negative groups was statistically significant, and (P= 0.028)3. this second and third generation virus carrier construction, slow ITIH4 gene shRNA slow virus carrier interference, a traditional method more efficient and reliable transfection of genetically modified method for the future, the gene targeting therapy lay a good foundation for the research.