Osteoclasts Induced By RANKL And M-CSF

In Chinese population, the incidence rate of periodontal disease arrives 70%. Alveolar bone resorption and formation of periodontal pocket are considered to be cardinal of the periodontal disease. Alveolar bone resorption is the major reason of dysmasesia and dyspepsia. Osteoclasts are essential cells for bone erosion in periodontal disease. Produce the pure populations of in vitro-generated osteoclasts that are required for biochemical and molecular analyses and also are essential for study the function of bone resorption Set up the model of osteoclasts is the first procedure of investigate bone resorption.Objective:Setting up the model of osteoclasts produce the pure populations of in vitro-generated osteoclasts. To investigate the effects of macrophage of colony-stimulating factor (M-CSF) and receptor activator of Nuclear factor-κB ligand (RANKL) on the differentiation and formation of induce RAW264.7 line.Methods:The RAW264.7 cells were treated with M-CSF and RANKL for several days. Observe the shapes of cells, Osteoclast marker-gene expression and TRAP staining. Bone resorption experiment were used to identify the osteoclasts.Result:The TRAP positive cell were induce from RAW264.7 cells afer treated with M-CSF and RANKL for 1 day. The positive multinuclear cells were induced after 3days. Different generations of cells express Osteoclast's marker-gene. Plenty of resorption pits formed on bone slices after 10days of induction.Conclusion:The RAW264.7cells in the presence of M-CSF and RANKL can successfully be induced to osteoclasts and this characteristics can be generated. Objective:To investigate efficiency of mandibular molars with furcation defects after separating roots and prosthesis with root canal thrapy,periodontal initial therapy.Methods:6 mandibular molars withⅢorⅣdegree furcation defects after canal therapy and periodontal initial therapy. then treated with roots separation and fixed prosthesis after 1 month.Results:Followed up and evaluated within 6 months-3 years, all molars had good masticatory function.Conclusion:Separating roots is an efficient method to conservative molars with furcation defects and recover masticatory function.