| ObjectivesSerum amyloid A,is a protein with high speciality, representating a family which relative molecular weight is about 12000. Some investigations told us that this pretein maybe have something to do with fatness and insulin resistance.High glucose level in blood could make fattiness cells secrete more SAA in vitro.SAA is likely to be a crucial fattiness cell factor which contact the fatness and relative metabolic diseases.To explore the relationship between SAA level and the fetal weight,and to analysis the significance of using SAA as a standard for estimating the nutritious condition of newborns,and to study the expression of serum amyloid A in the patients with gestation diabetes mellitus, we examined the SAA level both in umbilical cord blood of newborns' and plasma of pregnant women's.Methods1. The case control study was designed to analyze 40 cases of the patients with gestation diabetes mellitus (GDM).they all accord with these standards as followed: meet the gestation diabetes mellitus diagnostic normalization, pregnancy more than 37 weeks gestion, single fetus, and without other complications. There were 17 neonates with birth weight more than 4000g,and 23 neonates with normal birth weight.Save these pregnants'serum as case group.Collect 50 pregnants with the same gestation age and gravidity times,single fetus, pregnancy more than 37weeks gestion,and without complications. There were 20 neonates with birth weight more than 4000g,and 30 neonates with normal birth weight.Save these pregnants'serum as control group.SAA concentrations were determined by enzyme linked immunosorbent assay (ELISA)2.The neonates were divided into 4 groups,they were:group A, GDM pregnant women with their babies'birth weight over 4000g; group B, GDM pregnant women with their babies'birth weight were within the normal range; group C,Healthy pregnant women with their babies'birth weight over 4000g; group D, Healthy pregnant women with their babies'birth weight were within the normal range.SAA concentrations in umbilical cord blood were determined by enzyme linked immunosorbent assay(ELISA).Analyze the relationships between SAA and gestation diabetes mellitus as well as fetal weight.Results1. The SAA concentration in the plasma of pregnants with GDM was higher than that in the healthy pregnants.There was statistical significance(t=3.19, P<0.05).2.The SAA concentration in the neonates with birth weight over 4000g delivered by pregnants with GDM was higher than that in the neonates with normal birth weight delivered by pregnants with GDM.There was statistical significance(t=28.69, P <0.05); SAA concentration in the neonates with birth weight more than 4000g delivered by healthy pregnants was higher than that in the neonate with normal birth weight delivered by healthy pregnants.There was statistical significance(t=6.41, P <0.05); Among the neonates with birth weight more than 4000g,SAA in the neonates delivered by pregnants with GDM was higher than that in the neonates delivered by healthy pregnants. There was statistical significance(t=11.31, P<0.05); Among the neonates with normal birth weight,SAA concentration in the neonates delivered by pregnants with GDM was higher than that in the neonates delivered by healthy pregnants.There was not statistical significance(t=0.40, P>0.05)ConclusionOur study shows that venous blood of pregnant women is related with GDM. SAA content of neonate umbilical blood is related with macrosomia, now lots of epidemiologic studies find out that in obesity, insulin resistance, metabolize syndrome and type two diabetes patients, SAA level increases, in the future the risk whether SAA content of umbilical blood or venous blood of pregnant women can be used to predict newborns get fat, insulin resistance, metabolize syndrome and type two diabetes needs to be futher studied.During pregnant period, women need obstetric examination. Doctors should advise the pregnant women who may have macrosomia or get too much bodyweight to have appropriate activity, proper diet, nutrition equilibrium, make menu as everyday's caloric value. To middle, late pregnancy period, doctors combine with pregnant women height, bodyweight, uteral height, abdominal circumference, color dopplar to find pregnant women who may have macrosomia, give them advice, especially proper diet, nutrition equilibrium, advoidding taking overmuch food, reducing birth of macrosomia, reducing incidence of junior and adults's fat, and reducing various metabolic diseases. Doctors should find out and treat GDM pregnant women as soon as possible, exercise properly, take food as pregnant GDM diet, therapy with insulin when necessary, and survey blood sugar, make blood sugar stable, control fetal bodyweight. Maybe through SAA pathway to reduce insulin resistance, reduce sugar metabolism, improve mother-fetal prognosis, however this need further study to prove. |
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