The Effect Research Of TPO On M07e Cells Line In Vitro

[Background and Objective] Myelosuppression, a common complication of radiotherapy and chemotherapy in tumor treatment, is a main reason of dose restriction. Erythroproetin (EPO) and granulocyte colony stimulating factor (G-CSF) have already been widely used for the patients of anemia and granulocytopenia, and have got a good effect. The transfusion of blood platelets is a leading method for the patients of thrombocytopenia, but it still has some unavoidable deficit. National and international clinical researches showed TPO could decrease the degree of thrombocytopenia after chemotherapy, could shorten the time of thrombocytopenia, and could accelerate the speed of blood platelets to a normal level. It has a great significance of using TPO instead of blood platelets transfusion. Meanwhile, it will also decrease the cost and mortality rate. Nowadays, TPO is often given to the patients, and the peak value is in the day 12 to 14 after chemotherapy, whereas the blood platelet are decreased in the day 10 to 14 after using some chemotherapy drugs. The use of TPO after chemotherapy will not get a good result in some regimens, which often cause the early thrombocytopenia. Whether the minimum value of blood platelets will be increased when using TPO befor chemotherapy are in discussion. In this research, the influence of TPO on MO7e, and the different sequence of TPO for MO7e are discussed, so as to mimic the clinical use of TPO, and provide some foundation for the use of TPO.[Methods] Choose MO7e cells line instead of megakeryocyties.1)The detection of effect of TPO on MO7e by MTT and flow cytometry.2) The different sequence of TPO effect on MO7e before and after chemotherapy.3) IC50 of TPO compared with carboplatin was caculated in MO7e by MTT.4) IC50 of TPO compared with carboplatin was caculated in MO7e by MTT.[Results]TPO could promote the proliferation of MO7e cells, the proliferation is more obvious when the concentration increase.2) The use of TPO at 48h before chemotherapy is preferred to the use of TPO after chemotherapy (P＜0.05).3) The inhibition rate of carboplatin on M07e cells is decreased when TPO is added.4) The inhibition rate of carboplatin on M07e cells is far lower than the inhibition of carboplatin on MDA-MB-453 cells.[Conclusion] 1) TPO could promote the proliferation of megakeryocyties, the effect is line with the concentration.2) The use of TPO at 48h before chemotherapy is preferred to the use of TPO after chemotherapy.3) TPO could decrease the killing effect of carboplatin on M07e.4) The killing effect of breast cancer cells is higher than the megakeryocyties in the same concentration of carboplatin.