Effect Of Qingkailing Injection After Intracerebral Hemorrhage And Minimally Invasive Hematoma Aspiration In Rabbit

Objective:In this experiment,The intracerebral hematoma model was developed by the repetitious autoblood injection method.using minimally invasive hematoma aspiration and qingkailing injection to comprehensively examine the change of histopathological,Brain water content(BWC), Neurological deficits to evaluate the effets of qingkailing injection and minimally invasive hematoma aspiration methods on the process of brain injury.To explore rational administration after hematoma aspiration.Methods:64 healthy rabbits which ignore gender were assigned randomly to 4 groups,slam-operation group(group A,n=4),intracerebral hemorrhage(ICH)group(group B,n=20),hematoma aspiration group(group C,n=20)hematoma aspiration and qingkailing group(group D,n=20),The last three groups was further divided into groups of 6 hours,24 hours,3 days,7 days(n=5 in each group).The intracerebral hematoma model was developed by the repetitious injection method,we infused 0.3ml of fresh unclotted autologous blood into the right ganglia in rabbits.the method of qingkailing injection intervention:the rabbits were given to qingkailing injection at dose of 4ml/kg/d by the dorsal rabbit ear vein after intracerebral hemorrhage.the method of hematoma aspiration:2500u urokinase(dissolved by 50ul saline)was injected hematoma cavity after 4h,then 2h later,the clot was aspirated.The immunohistochemical(IHC)expression of AQP-4 after intracerebral hemorrhage were determined using immuno-histochemistry and brain water content measured by using the dry-wet weight assay, neurological deficits were examined with modified Bederson's. Results:The BWC of group B increased gradually from 6h after ICH and peaked at day 3,afterwards,it began to reduce,it was obviously higher than group A at day 7(P<0.05).the BWC of group C was obviously higher than group A and group B at 6 hours(P<0.05)and peaked at 24h,there is no difference between group C and group B,afterwards,it began to reduce, to control with group B,the BWC of group C was obviously reduced at day 3 and 7(P<0.05),there was no obvious difference between the group of group C and group A at day 7.To control with group C,the BWC of group D was obviously reduced at 24h and day 3(P<0.05),there was no difference between them at 6h and day 7.Neurologic functional recovery began at 24h after operation,but there was no obvious difference between the three groups(P>0.05).neurological defect score of group B decreased at 3d,but the score of group C,group D increased markedly,there were more significant differences compared with groupB,the score of group D was higher than the score of group C and there were significant differences between them(P<0.05).neurologic function of group C,group D recovered mainly at 7d,there were significant differences compared with group B(P<0.01)The AQP-4 expression around hematoma increased at 6h after ICH and reached a peak at day 3,afterwards,the AQP-4 expression around hematoma reduced gradually,it reduced more at 7d.Compared with the AQP-4 expression of group B,group C and group D,there is no difference at 6h,24h.Compared with group B,the AQP-4 expression of group C,group D around hematoma reduced at day 3(P<0.05),the AQP-4 expression of group D reduced more than group C(P<0.01).at 7d the AQP-4 expression of group C,group D is lower obviously than that of group B(P<0.01),the AQP-4 expression of group D is lower than that of group C too(P<0.01)...