The Treatment Of Lactobacillus Preparation On Bacterial Vaginosis And The Regulation Of Local Immune

Lactobacillus is a common gram-positive bacteria which is beneficial to human. It is one of the security and normal flora in vaginal and gastrointestinal tract of human. A number of studies have shown that Lactobacillus have a role in resistanting the growth and reproduction of endogenous and exogenous pathogenic in the vagina. Vaginal lactobacilli has important physiological functions in decreasing pH, maintaining vaginal cleanliness, keeping the ecological balance of the vaginal micro.Lactobacillus is a class of important microorganisms which has a good prospect. Lactobacillus Preparation is a live bacteria made of artificial propagation in vitro which is isolated from human or animal vaginal lactobacillus, and ultimately playing its natural physiological function back to the body, adjusting the vaginal flora, maintaining ecological balance, so as to achieve the purpose of preventing the disease. It is already proved that it has more obvious results in preventing and treating vaginal flora imbalance caused by a variety of diseases.Bacterial vaginosis (BV) is the most common and complex clinical infectious diseases in gynecological which is characterized by changes in proportion of the normal vaginal flora, its pathogenesis is not clear yet.In recent years, researches in the lower genital tract infection and cytokines have become popular, especially the relationship between diseases and changes of local immune environment of the vagina is highly valued.Currently, the antibiotic is still the main therapy for these diseases, but the result is not satisfactory:the treatment is not thorough, easy to producing drug resistance, easy to relapse. Microbial therapy is quite concerned in recent years, it added lactobacilli in the vagina to inhibit the growth of many pathogens, improve the environment of the vagina and restore the ecological balance of the vaginal micro, so as to achieve the therapeutic effect.In addition, it is safe and non-toxic side effects that avoids all the disadvantages of antibiotic therapy fundamentally.As one of the probioticses, Lactobacillus have a certainly results in treating vaginal infections in clinical. It has been widely recognized in maintaining health of human and regulating the immune function.A variety of lactobacillus lactis can regulate the immune system, but the specific mechanisms of these changes has not been determined.Objective:Detect the immune cytokines levels of normal women and patients with BV before and after treatment. Discuss the effect of Lactobacillus preparation on reproductive tract in immunity and lay a theoretical basis of Lactobacillus preparation for clinical treatment; Discuss the relationship between bacterial vaginosis and vaginal mucosal immunity and provide some clinical trial data for studying the relationship between vaginal mucous membrane and vaginal mucosal immune barrier.Methods:Evaluate the efficacy of Lactobacillus Preparation treating the patients with bacterial vaginosis, and detect the level of cytokines sIgA, IL-2, IL-13 in the vaginal fluid of patients with bacterial vaginosis and normal healthy women by enzyme-linked immunosorbent assay method that called ELISA.Results:1)The level of sIgA in vaginal fluid of each group:The level of sIgA of BV group before treatment was significantly higher than the control group (p<0.01); The level of sIgA of BV group after treatment was significantly lower than BV group before treatment (p<0.05); The level of sIgA of BV group after treatment was slightly lower than control group (p>0.05); 2) The level of IL-2 in vaginal fluid of each group:The level of sIgA of BV group before treatment was significantly lower than the control group (p<0.01); The level of slgA of BV group after treatment was higher than BV group before treatment (p<0.05); The level of slgA of BV group after treatment was slightly higher than control group (p>0.05); 3) The level of IL-13 in vaginal fluid of each group:The level of sIgA of BV group before treatment was significantly higher than the control group (p<0.01); The level of sIgA of BV group after treatment was significantly lower than BV group before treatment (p<0.05); The level of sIgA of BV group after treatment was slightly lower than control group (p>0.05);Conclusion:Lactobacillus preparation has a good effect on treating bacterial vaginosis; Bacterial vaginal patients have changed in local immune in vaginal; Lactobacillus preparation can regulate the patients' local immune with bacterial vaginosis.