The Study Of The Influence To Different Intensity Exercise On Mice CD4~+CD25~+ Regulatory T Cell And The Immune Effects Of HBV DNA Vaccine

ObjectivesTo explore the machanisms of the different intensity exercise on mice CD4~+CD25~+regulatory T cells (Treg) and HBV DNA vaccine (pVAX-S2) and provide a theoretical basis for immune suppression caused by High intensity exercise and appropriate exercise intensity for health promotion as well as different intensity exercise on the effect of HBV DNA vaccine.MethodsThe 96 C57BL/6 mice were randomly divided into four groups: normal control group (Na?ve), moderate-intensity exercise group (MIE), high-intensity exercise group (HIE), treadmill control group (CK), and build models of different intensity exercise. Half of the mice in each group in the sports section immuned HBV DNA vaccine pVAX-S2 by intramuscular injection on the 0, 14 and 28 day. Drawn were sacrificed after 6 weeks. Are not immunzed mice use RT-PCR detection of cytokine gene IL-12p40(for IL-12), TGF-β, IL-35, IL-2 expression; flow cytometry technique testing cytokines IL-10, IFN-γ, the total splenic lymphocyte IL-2Rα(CD25), CD4~+T lymphocyte subsets in IL-2Rα(CD25), CD4~+CD25~+Treg cell-specific marker Foxp3, and CD4~+CD25~+Treg effector molecule of IL-10 expression. the mice immunized with HBV DNA vaccine pVAX-S2 use flow cytometry technique to detect T lymphocyte proliferation, T lymphocyte-specific antigen peptide toxicity and CD4~+ and CD8~+ lymphocyte subsets in IFN-γand CD4~+T lymphocyte subsets, IL-4 expression. By quantitative ELISA detection of hepatitis B antibody IgG..Results1. HIE could significantly up-regulated the anti-inflammatory cytokines expression such as IL-10,TGF-βand IL-35 while down-regulated the pro-inflammatory cytokines expressions, including IL-2, IL-12 and IFN-γ. However, MIE did not change the IL-10, TGF-β, and IL-35 expressions but significantly increase the expression of IL-2, IL-12 and IFN-γ. 2. Both high and moderate intensity exercises could significantly enhance IL-2 receptor (CD25) expressions.3. HIE significantly increased CD4~+ T lymphocyte subsets in IL-2Rα(CD25), CD4~+CD25~+Treg cell-specific marker Foxp3, and CD4~+CD25~+Treg effect or molecule of the expression of IL-10 However, MIE did not change the expression of CD4~+T lymphocyte subsets in IL-2Rα(CD25), CD4~+CD25~+Treg cell-specific marker Foxp3, and CD4~+CD25~+Treg effector molecule of the expression of IL-10.4. MIE could significantly enhanced cellular immune function such as increasing IFN-γexpression in CD4~+ and CD8~+ T cells, T-lymphocyte proliferation and antigen-specific T lymphocyte cytotoxic responses in the animal immunized with HBV DNA vaccine pVAX-S2. However, the cellular immune responseswere significantly impaired by HIE. Both moderate and high intensity exercise did not affect the production of IgG antibodies against hepatitis B and the expression of CD4~+T lymphocyte subsets IL-4.ConclusionsHigh intensity exercise might inhibit the cellular immune function in mice immunized with DNA vaccine by enhancing the CD4~+CD25~+Treg cells and anti-inflammatory patterns.Moderate intensity exercise had no significant effect on mouse CD4~+CD25~+Treg, mainly by increasing the expression of pro-inflammatory factors to enhance the body's immune function, and so improve the cellular immune function in mice immunized with DNA vaccine...